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Yersinia enterocolitica YopQ: strain-dependent cytosolic accumulation and post-translational secretion

Janja Trek^a,b,1

Max von Pettenkofer-Institut, Pettenkoferstr. 9a, D-80336 Munich, Germany¹

Author for correspondence: Jürgen Heesemann. Tel: +49 89 5160 5200. Fax: +49 89 5160 5202. e-mail: heesemann{at}m3401.mpk.med.uni-muenchen.de

YopQ in Yersinia enterocolitica (YopK in Yersinia pseudotuberculosis) is a type III secreted protein required for virulence of yersiniae. In this study YopQ expression, secretion and nucleotide sequences of the corresponding yopQ gene from different yersinia strains were analysed. The cytosolic accumulation differed significantly among serotypes of Y. enterocolitica. These differences might be attributable to variations in the nucleotide sequence and their consequences on mRNA secondary structure. An mRNA signal hypothesis has been proposed for YopQ, predicting the coupling of translation and secretion via an mRNA signal. This hypothesis claims a strictly co-translational secretion of YopQ without its intracellular accumulation. The presence of YopQ in the cytosol, even with a closed secretion apparatus, is demonstrated. Moreover, post-translational secretion of YopQ could be demonstrated. These findings do not support the mRNA signal hypothesis for co-translational secretion.

Keywords: YopQ-GFP accumulation, yopQ/yopK sequences, mRNA secondary structures

Abbreviations: BHI, brain–heart infusion; Cm, chloramphenicol; GFP, green fluorescent protein; GST, glutathione S-transferase

The GenBank/EMBL accession numbers for the sequences reported in this paper are AJ421529 [yopQ gene fragment from Y. enterocolitica WA-314 (O:8)] and AJ421530 [yopQ gene fragment from Y. enterocolitica Y-108-P (O:3)].

^a These authors contributed equally to this work.

^b Present address: University of Ljubljana, Institute of Microbiology and Microbial Biotechnology, Groblje 3, SI-1230 Domale, Slovenia.

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