| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Research Paper |
School of Biomedical and Life Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK1
Author for correspondence: Simon F. Park. Tel: +44 1483 879024. Fax: +44 1483 300374. e-mail: s.park{at}surrey.ac.uk
The expression of a wide variety of physiological functions in many bacterial species is modulated by quorum sensing, a population-dependent signalling mechanism that involves the production and detection of extracellular signalling molecules. The genome sequence of Campylobacter jejuni NCTC 11168 contains a gene encoding an orthologue of LuxS, which is required for autoinducer-2 (AI-2) production in other bacterial species, but does not contain genes predicted to encode any known acyl-homoserine lactone synthetase. This study demonstrates that C. jejuni produces functional AI-2 activity through the ability of cell-free extracts to specifically induce bioluminescence in Vibrio harveyi BB170, a reporter strain for quorum-sensing system 2. Production of this signalling compound was shown to be dependent upon the product of the C. jejuni luxS gene (Cj1198). While the luxS mutant showed comparable growth rate, resistance to oxidative stress and ability to invade Caco-2 cell monolayers to the parental strain, it exhibited decreased motility haloes in semisolid media, suggesting a role for quorum sensing in the regulation of motility.
Keywords: LuxS, autoinducer-2, camplylobacters
Abbreviations: AI-1/2, autoinducer-1/2; HSL, homoserine lactone
This article has been cited by other articles:
|
|
 |
|
  B. A. Rader, S. R. Campagna, M. F. Semmelhack, B. L. Bassler, and K. Guillemin The Quorum-Sensing Molecule Autoinducer 2 Regulates Motility and Flagellar Morphogenesis in Helicobacter pylori J. Bacteriol., September 1, 2007; 189(17): 6109 - 6117. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Reeser, R. T. Medler, S. J. Billington, B. H. Jost, and L. A. Joens Characterization of Campylobacter jejuni Biofilms under Defined Growth Conditions Appl. Envir. Microbiol., March 15, 2007; 73(6): 1908 - 1913. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Phongsisay, V. N. Perera, and B. N. Fry Expression of the htrB gene is essential for responsiveness of Salmonella typhimurium and Campylobacter jejuni to harsh environments Microbiology, January 1, 2007; 153(1): 254 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Krin, N. Chakroun, E. Turlin, A. Givaudan, F. Gaboriau, I. Bonne, J.-C. Rousselle, L. Frangeul, C. Lacroix, M.-F. Hullo, et al. Pleiotropic Role of Quorum-Sensing Autoinducer 2 in Photorhabdus luminescens. Appl. Envir. Microbiol., October 1, 2006; 72(10): 6439 - 6451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Challan Belval, L. Gal, S. Margiewes, D. Garmyn, P. Piveteau, and J. Guzzo Assessment of the Roles of LuxS, S-Ribosyl Homocysteine, and Autoinducer 2 in Cell Attachment during Biofilm Formation by Listeria monocytogenes EGD-e Appl. Envir. Microbiol., April 1, 2006; 72(4): 2644 - 2650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, J. Li, J. C. March, J. J. Valdes, and W. E. Bentley luxS-Dependent Gene Regulation in Escherichia coli K-12 Revealed by Genomic Expression Profiling J. Bacteriol., December 15, 2005; 187(24): 8350 - 8360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. Snelling, J. P. McKenna, D. M. Lecky, and J. S. G. Dooley Survival of Campylobacter jejuni in Waterborne Protozoa Appl. Envir. Microbiol., September 1, 2005; 71(9): 5560 - 5571. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Kaper and V. Sperandio Bacterial Cell-to-Cell Signaling in the Gastrointestinal Tract Infect. Immun., June 1, 2005; 73(6): 3197 - 3209. [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, Y. Hashimoto, C.-Y. Tsao, J. J. Valdes, and W. E. Bentley Cyclic AMP (cAMP) and cAMP Receptor Protein Influence both Synthesis and Uptake of Extracellular Autoinducer 2 in Escherichia coli J. Bacteriol., March 15, 2005; 187(6): 2066 - 2076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. B. Xavier and B. L. Bassler Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli J. Bacteriol., January 1, 2005; 187(1): 238 - 248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Loh, M. H. Forsyth, and T. L. Cover Growth Phase Regulation of flaA Expression in Helicobacter pylori Is luxS Dependent Infect. Immun., September 1, 2004; 72(9): 5506 - 5510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. T. Elvers, G. Wu, N. J. Gilberthorpe, R. K. Poole, and S. F. Park Role of an Inducible Single-Domain Hemoglobin in Mediating Resistance to Nitric Oxide and Nitrosative Stress in Campylobacter jejuni and Campylobacter coli J. Bacteriol., August 15, 2004; 186(16): 5332 - 5341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Joyce, A. Kawale, S. Censini, C. C. Kim, A. Covacci, and S. Falkow LuxS Is Required for Persistent Pneumococcal Carriage and Expression of Virulence and Biosynthesis Genes Infect. Immun., May 1, 2004; 72(5): 2964 - 2975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Jones and M. J. Blaser Detection of a luxS-Signaling Molecule in Bacillus anthracis Infect. Immun., July 1, 2003; 71(7): 3914 - 3919. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
