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Microbiology 148 (2002), 1553-1559
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Microbiology (2002), 148, 1553-1559.
© 2002 Society for General Microbiology

Research Paper

The regulation of Enzyme IIAGlc expression controls adenylate cyclase activity in Escherichia coli

Evelyne Krin1, Odile Sismeiroa,1, Antoine Danchin1 and Philippe N. Bertinb,1

Unité de Génétique des Génomes Bactériens, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France1

Author for correspondence: Evelyne Krin. Tel: +33 01 40 61 35 56. Fax: +33 01 45 68 89 48. e-mail: ekrin{at}pasteur.fr

During the last few years, several genes, such as pap, bgl and flhDC, have been shown to be coregulated by the histone-like nucleoid-structuring (H-NS) protein and the cyclic AMP-catabolite activator protein (cAMP/CAP) complex, suggesting an interaction between both systems in the control of some cellular functions. In this study, the possible effect of H-NS on the cAMP level was investigated. In a CAP-deficient strain, the presence of an hns mutation results in a strong reduction in the amount of cAMP, due to a decrease in adenylate cyclase activity. This is caused by the reduced expression of crr, which encodes the Enzyme IIAGlc of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS), from its specific P2 promoter. This leads to a twofold reduction in the global amount of Enzyme IIAGlc, the adenylate cyclase activator, responsible for the decrease in adenylate cyclase activity observed in the hns crp strain.

Keywords: catabolite repression, cya, regulatory network

Abbreviations: CAP, catabolite activator protein; H-NS, histone-like nucleoid-structuring; PTS, phosphoenolpyruvate:carbohydrate phosphotransferase system

a Present address: Genopole, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France.

b Present address: Laboratory of Microbiology, Radioactive Waste & Clean-up Division, SCK/CEN, Boeretang 200, 2400 Mol, Belgium.




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