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Research Paper |
B-dependent general stress regulons of Bacillus subtilis
Department of Microbiology and Immunology, The Medical School, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK1
Author for correspondence: Colin R. Harwood. Tel: +44 191 222 7708. Fax: +44 191 222 7736. e-mail: Colin.Harwood{at}ncl.ac.uk
When Bacillus subtilis is subjected to phosphate starvation, the Pho and 
B-dependent general stress regulons are activated to elicit, respectively, specific and non-specific responses to this nutrient-limitation stress. A set of isogenic mutants, with a ß-galactosidase reporter gene transcriptionally fused to the inactivated target gene, was used to identify genes of unknown function that are induced or repressed under phosphate limitation. Nine phosphate-starvation-induced (psi) genes were identified: yhaX, yhbH, ykoL and yttP were regulated by the PhoP–PhoR two-component system responsible for controlling the expression of genes in the Pho regulon, while ywmG (renamed csbD), yheK, ykzA, ysnF and yvgO were dependent on the alternative sigma factor B, which controls the expression of the general stress genes. Genes yhaX and yhbH are unique members of the Pho regulon, since they are phosphate-starvation induced via PhoP–PhoR from a sporulation-specific E promoter or a promoter that requires the product of a E-dependent gene. Null mutations in key regulatory genes phoR and sigB showed that the Pho and B-dependent general stress regulons of Bacillus subtilis interact to modulate the levels at which each are activated.
Keywords: stress response, psi genes, phosphate starvation, regulatory networks
Abbreviations: APase, alkaline phosphatase; BFA, Bacillus subtilis functional analysis; HPM, high-phosphate medium; LPM, low-phosphate medium; B-GS, B-dependent general stress
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