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Microbiology 148 (2002), 1821-1831
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Microbiology (2002), 148, 1821-1831.
© 2002 Society for General Microbiology

Research Paper

Differential cross-complementation patterns of Escherichia coli and Neisseria gonorrhoeae RecA proteins

Elizabeth A. Stohl1, Leslie Blount1 and H. Steven Seifert1

Department of Microbiology-Immunology, The Feinberg School of Medicine, Northwestern University, 303 E Chicago Avenue, Chicago, IL 60611, USA1

Author for correspondence: H. Steven Seifert. Tel: +1 312 503 9788. Fax: +1 312 503 1339. e-mail: h-seifert{at}northwestern.edu

The Escherichia coli RecA protein is one of the best-studied enzymes, but less is understood about how RecA homologues of other species are similar to or different from the E. coli RecA. In the Gram-negative pathogen Neisseria gonorrhoeae (the gonococcus; Gc), the causative agent of gonorrhoea, RecA is involved in DNA transformation, pilin antigenic variation, and DNA repair. By expressing the recA genes from Gc and E. coli under control of lac regulatory sequences in E. coli, the authors have shown that the Gc RecA fully complements an E. coli recA mutant for homologous recombination, but only partially complements for survival to DNA damage. By expressing similar constructs in Gc, it was shown that the E. coli RecA complements for pilin antigenic variation, partially complements for DNA transformation, but does not complement for survival to DNA damage, suggesting that species-specific interactions are important for DNA repair, but not for homologous recombination. Co-expression of the E. coli recA and recX genes in Gc suggests that in this heterologous system RecX modulates RecA-mediated processes.

Keywords: DNA repair, pilus antigenic variation, homologous recombination, recX

Abbreviations: Gc, Neisseria gonorrhoeae




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