| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Research Paper |
Laboratoire de Microbiologie, ENSBANA, 1 Esplanade Erasme, 21000 Dijon, France1
Laboratoire de Microbiologie, Faculté de Pharmacie, 7 boulevard Jeanne d’Arc, 21033 Dijon Cedex, France2
Author for correspondence: Jean-Paul Lemaître. Tel:+33 3 80 39 66 79. Fax: +33 3 80 39 66 40. e-mail: jplemait{at}u-bourgogne.fr
Two human faeces carriage isolates of Listeria monocytogenes (H1 and H2) were compared to reference strains (ScottA and LO28) with regard to their lethality in 14-day-old chick embryos, their haemolytic and phospholipase (phosphatidylcholine-phospholipase C and phosphatidylinositol-phospholipase C) activities and their invasiveness towards Caco-2 cells. Experimental infection of chick embryos allowed discrimination of the strains into those exhibiting high virulence (ScottA and H2), those exhibiting slightly attenuated virulence (LO28) and those exhibiting low virulence (H1). A similar percentage mortality and time to death for embryos was observed when they were infected with H2 as was seen with infection by the reference strain ScottA. Therefore, human carriage strain H2 was considered potentially pathogenic. In contrast to H2 and ScottA, H1 exhibited low virulence. Using the tissue-culture cell-line model, it was found that carriage strain H1 was unable to enter Caco-2 cells efficiently, even though it was similar to the virulent strains in terms of the enzymic activities involved in pathogenicity. Detection of the internalins InlA and InlB, involved in the internalization of L. monocytogenes in the host cells, by immunoblot indicated that a truncated form of InlA was produced by H1. Taken together, these data provide a starting point for the study of the behaviour of two types of human faeces carriage strains and their characterization.
Keywords: virulence, internalin, chick-embryo assay, Caco-2 cell-culture assay
Abbreviations: ACA, acriflavine–ceftazidime agar; Pc-PLC, phosphatidylcholine-specific phospholipase C; Pi-PLC, phosphatidylinositol-specific phospholipase C
The GenBank accession number for the sequence reported in this paper is AF468816.
a Present address: Equipe Aliment et Cancer UMR INRA-ENVT 1089, Toulouse, France.
This article has been cited by other articles:
|
|
 |
|
  V. Lopez, S. Ortiz, A. Corujo, P. Lopez, D. Poza, J. Navas, R. Moreno, and J. V. Martinez-Suarez Different Contamination Patterns of Lineage I and II Strains of Listeria monocytogenes in a Spanish Broiler Abattoir Poult. Sci., September 1, 2008; 87(9): 1874 - 1882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Severino, O. Dussurget, R. Z. N. Vencio, E. Dumas, P. Garrido, G. Padilla, P. Piveteau, J.-P. Lemaitre, F. Kunst, P. Glaser, et al. Comparative Transcriptome Analysis of Listeria monocytogenes Strains of the Two Major Lineages Reveals Differences in Virulence, Cell Wall, and Stress Response Appl. Envir. Microbiol., October 1, 2007; 73(19): 6078 - 6088. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. H. Orsi, D. R. Ripoll, M. Yeung, K. K. Nightingale, and M. Wiedmann Recombination and positive selection contribute to evolution of Listeria monocytogenes inlA Microbiology, August 1, 2007; 153(8): 2666 - 2678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. S. Felicio, T. Hogg, P. Gibbs, P. Teixeira, and M. Wiedmann Recurrent and Sporadic Listeria monocytogenes Contamination in Alheiras Represents Considerable Diversity, Including Virulence-Attenuated Isolates Appl. Envir. Microbiol., June 15, 2007; 73(12): 3887 - 3895. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lecuit, J. L. Sonnenburg, P. Cossart, and J. I. Gordon Functional Genomic Studies of the Intestinal Response to a Foodborne Enteropathogen in a Humanized Gnotobiotic Mouse Model J. Biol. Chem., May 18, 2007; 282(20): 15065 - 15072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. F. Ducey, B. Page, T. Usgaard, M. K. Borucki, K. Pupedis, and T. J. Ward A Single-Nucleotide-Polymorphism-Based Multilocus Genotyping Assay for Subtyping Lineage I Isolates of Listeria monocytogenes Appl. Envir. Microbiol., January 1, 2007; 73(1): 133 - 147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Moroni, E. Kheadr, Y. Boutin, C. Lacroix, and I. Fliss Inactivation of Adhesion and Invasion of Food-Borne Listeria monocytogenes by Bacteriocin-Producing Bifidobacterium Strains of Human Origin Appl. Envir. Microbiol., November 1, 2006; 72(11): 6894 - 6901. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Liu Identification, subtyping and virulence determination of Listeria monocytogenes, an important foodborne pathogen J. Med. Microbiol., June 1, 2006; 55(6): 645 - 659. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Challan Belval, L. Gal, S. Margiewes, D. Garmyn, P. Piveteau, and J. Guzzo Assessment of the Roles of LuxS, S-Ribosyl Homocysteine, and Autoinducer 2 in Cell Attachment during Biofilm Formation by Listeria monocytogenes EGD-e Appl. Envir. Microbiol., April 1, 2006; 72(4): 2644 - 2650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Nightingale, K. Windham, K. E. Martin, M. Yeung, and M. Wiedmann Select Listeria monocytogenes Subtypes Commonly Found in Foods Carry Distinct Nonsense Mutations in inlA, Leading to Expression of Truncated and Secreted Internalin A, and Are Associated with a Reduced Invasion Phenotype for Human Intestinal Epithelial Cells Appl. Envir. Microbiol., December 1, 2005; 71(12): 8764 - 8772. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Roche, P. Gracieux, E. Milohanic, I. Albert, I. Virlogeux-Payant, S. Temoin, O. Grepinet, A. Kerouanton, C. Jacquet, P. Cossart, et al. Investigation of Specific Substitutions in Virulence Genes Characterizing Phenotypic Groups of Low-Virulence Field Strains of Listeria monocytogenes Appl. Envir. Microbiol., October 1, 2005; 71(10): 6039 - 6048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. K. Nightingale, K. Windham, and M. Wiedmann Evolution and Molecular Phylogeny of Listeria monocytogenes Isolated from Human and Animal Listeriosis Cases and Foods J. Bacteriol., August 15, 2005; 187(16): 5537 - 5551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Olier, D. Garmyn, S. Rousseaux, J.-P. Lemaitre, P. Piveteau, and J. Guzzo Truncated Internalin A and Asymptomatic Listeria monocytogenes Carriage: In Vivo Investigation by Allelic Exchange Infect. Immun., January 1, 2005; 73(1): 644 - 648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Rousseaux, M. Olier, J. P. Lemaitre, P. Piveteau, and J. Guzzo Use of PCR-Restriction Fragment Length Polymorphism of inlA for Rapid Screening of Listeria monocytogenes Strains Deficient in the Ability To Invade Caco-2 Cells Appl. Envir. Microbiol., April 1, 2004; 70(4): 2180 - 2185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Liu, A. J. Ainsworth, F. W. Austin, and M. L. Lawrence Characterization of virulent and avirulent Listeria monocytogenes strains by PCR amplification of putative transcriptional regulator and internalin genes J. Med. Microbiol., December 1, 2003; 52(12): 1065 - 1070. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. W. Jaradat and A. K. Bhunia Adhesion, Invasion, and Translocation Characteristics of Listeria monocytogenes Serotypes in Caco-2 Cell and Mouse Models Appl. Envir. Microbiol., June 1, 2003; 69(6): 3640 - 3645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Olier, F. Pierre, S. Rousseaux, J.-P. Lemaitre, A. Rousset, P. Piveteau, and J. Guzzo Expression of Truncated Internalin A Is Involved in Impaired Internalization of Some Listeria monocytogenes Isolates Carried Asymptomatically by Humans Infect. Immun., March 1, 2003; 71(3): 1217 - 1224. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
