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Isolation of the patC gene encoding the cystathionine ß-lyase of Lactobacillus delbrueckii subsp. bulgaricus and molecular analysis of inter-strain variability in enzyme biosynthesis^a

Unité de Microbiologie et Génétique, UMR 5122, Université Claude Bernard-Lyon 1, bât. Lwoff, 10 rue Dubois, F-69622 Villeurbanne Cedex, France¹
Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blancs, CH-1000 Lausanne 26, Switzerland²

Author for correspondence: Dominique Aubel. Tel: +33 4 72 43 13 66. Fax: +33 4 72 43 26 86. e-mail: aubel{at}biomserv.univ-lyon1.fr

The patC gene encoding the cystathionine ß-lyase (CBL) of Lactobacillus delbrueckii subsp. bulgaricus NCDO 1489 was cloned and expressed in Escherichia coli. Overexpression of CBL complemented the methionine auxotrophy of an E. coli metC mutant, demonstrating in vivo that this enzyme functions as a CBL. However, PatC is distinguishable from the MetC CBLs by a low identity in amino acid sequence, a sensitivity to iodoacetic acid, greater thermostability and a lower substrate affinity. Homologues of patC were detected in the 13 Lb. delbrueckii strains studied, but only seven of them showed CBL activity. In constrast to CBL⁺ strains, all CBL-deficient strains analysed were auxotrophic for methionine. This supports the hypothesis that CBLs from lactobacilli are probably involved in methionine biosynthesis. Moreover, the results of this study suggest that post-transcriptional mechanisms account for the differences in CBL activities observed between strains of Lb. delbrueckii.

Keywords: amino acid catabolism, ß-cystathionase, PatC, lactic acid bacteria, cheesemaking

Abbreviations: CBL, cystathionine ß-lyase; DMDS, dimethyldisulfide; DMTS; dimethyltrisulfide; PLP, pyridoxal 5'-phosphate

^a The GenBank accession number for the sequence determined in this work is AF423071.

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