Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 148 (2002), 2045-2053
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ogunniyi, A. D.
Right arrow Articles by Paton, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ogunniyi, A. D.
Right arrow Articles by Paton, J. C.
Agricola
Right arrow Articles by Ogunniyi, A. D.
Right arrow Articles by Paton, J. C.
Microbiology (2002), 148, 2045-2053.
© 2002 Society for General Microbiology

Research Paper

The genes encoding virulence-associated proteins and the capsule of Streptococcus pneumoniae are upregulated and differentially expressed in vivo

A. David Ogunniyi1, Philippe Giammarinaro1 and James C. Paton1

Department of Molecular Biosciences, Adelaide University, Adelaide, South Australia 5005, Australia1

Author for correspondence: James C. Paton. Tel: +61 8 83035929. Fax: +61 8 83033262. e-mail: james.paton{at}adelaide.edu.au

The polysaccharide capsule of Streptococcus pneumoniae and several well-characterized virulence proteins are known to contribute to the pathogenesis of pneumococcal disease. However, there is a paucity of data on the expression of their respective genes in vivo. In this study, the relative abundance of the mRNA transcripts of the genes encoding pneumolysin (ply), pneumococcal surface protein A (pspA), pneumococcal surface antigen A (psaA) and choline-binding protein A (cbpA), and of the first gene of the capsular polysaccharide biosynthesis locus (cps2A), was measured in virulent type 2 pneumococci harvested from the blood of BALB/c mice at 12 h and 24 h following intraperitoneal infection. The mRNA levels were then compared, using relative quantitative RT-PCR, with those present in organisms grown in serum broth. The expression of ply was upregulated threefold at 12 h, and 10-fold at 24 h post-infection; the expression of pspA and psaA was upregulated threefold and fivefold, respectively, at 12 h post-infection. Interestingly, the expression of pspA was 36-fold higher at 24 h post-infection whereas the expression of cps2A was upregulated approximately fourfold at 12 and 24 h post-infection. However, cbpA mRNA levels remained comparable in vivo and in vitro. When organisms were grown in whole blood or THY broth, the relative expression of these genes in the two growth media also differed markedly. This work provides direct molecular evidence that known virulence-associated genes of S. pneumoniae are differentially expressed in vivo. Data on the relative expression of these genes in different growth media also suggests that the regulation of expression of these genes is highly complex and multifactorial.

Keywords: pneumococcus, pneumolysin, surface proteins, mRNA, quantitative RT-PCR

Abbreviations: PS, polysaccharide




This article has been cited by other articles:


Home page
 DNA ResHome page
M. Moscoso and E. Garcia
Transcriptional Regulation of the Capsular Polysaccharide Biosynthesis Locus of Streptococcus Pneumoniae: a Bioinformatic Analysis
DNA Res, June 1, 2009; 16(3): 177 - 186.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. R. Quin, Q. C. Moore III, J. A. Thornton, and L. S. McDaniel
Peritoneal Challenge Modulates Expression of Pneumococcal Surface Protein C during Bacteremia in Mice
Infect. Immun., March 1, 2008; 76(3): 1122 - 1127.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. K. Mahdi, A. D. Ogunniyi, K. S. LeMessurier, and J. C. Paton
Pneumococcal Virulence Gene Expression and Host Cytokine Profiles during Pathogenesis of Invasive Disease
Infect. Immun., February 1, 2008; 76(2): 646 - 657.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
L. J. Hathaway, P. Battig, and K. Muhlemann
In vitro expression of the first capsule gene of Streptococcus pneumoniae, cpsA, is associated with serotype-specific colonization prevalence and invasiveness
Microbiology, August 1, 2007; 153(8): 2465 - 2471.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
A. J. Standish, U. H. Stroeher, and J. C. Paton
The Pneumococcal Two-Component Signal Transduction System RR/HK06 Regulates CbpA and PspA by Two Distinct Mechanisms
J. Bacteriol., August 1, 2007; 189(15): 5591 - 5600.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
W. T. Hendriksen, N. Silva, H. J. Bootsma, C. E. Blue, G. K. Paterson, A. R. Kerr, A. de Jong, O. P. Kuipers, P. W. M. Hermans, and T. J. Mitchell
Regulation of Gene Expression in Streptococcus pneumoniae by Response Regulator 09 Is Strain Dependent
J. Bacteriol., February 15, 2007; 189(4): 1382 - 1389.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. D. Ogunniyi, M. Grabowicz, D. E. Briles, J. Cook, and J. C. Paton
Development of a Vaccine against Invasive Pneumococcal Disease Based on Combinations of Virulence Proteins of Streptococcus pneumoniae
Infect. Immun., January 1, 2007; 75(1): 350 - 357.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
G. K. Paterson, C. E. Blue, and T. J. Mitchell
Role of two-component systems in the virulence of Streptococcus pneumoniae.
J. Med. Microbiol., April 1, 2006; 55(Pt 4): 355 - 363.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. W. Johnston, D. E. Briles, L. E. Myers, and S. K. Hollingshead
Mn2+-Dependent Regulation of Multiple Genes in Streptococcus pneumoniae through PsaR and the Resultant Impact on Virulence
Infect. Immun., February 1, 2006; 74(2): 1171 - 1180.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
S. Bergmann and S. Hammerschmidt
Versatility of pneumococcal surface proteins
Microbiology, February 1, 2006; 152(2): 295 - 303.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
K. S. LeMessurier, A. D. Ogunniyi, and J. C. Paton
Differential expression of key pneumococcal virulence genes in vivo
Microbiology, February 1, 2006; 152(2): 305 - 311.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
W.-L. Ng, H.-C. T. Tsui, and M. E. Winkler
Regulation of the pspA Virulence Factor and Essential pcsB Murein Biosynthetic Genes by the Phosphorylated VicR (YycF) Response Regulator in Streptococcus pneumoniae
J. Bacteriol., November 1, 2005; 187(21): 7444 - 7459.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Thornton and L. S. McDaniel
THP-1 Monocytes Up-Regulate Intercellular Adhesion Molecule 1 in Response to Pneumolysin from Streptococcus pneumoniae
Infect. Immun., October 1, 2005; 73(10): 6493 - 6498.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. Y. Salim, D. G. Cvitkovitch, P. Chang, D. J. Bast, M. Handfield, J. D. Hillman, and J. C. S. de Azavedo
Identification of Group A Streptococcus Antigenic Determinants Upregulated In Vivo
Infect. Immun., September 1, 2005; 73(9): 6026 - 6038.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Hammerschmidt, S. Wolff, A. Hocke, S. Rosseau, E. Muller, and M. Rohde
Illustration of Pneumococcal Polysaccharide Capsule during Adherence and Invasion of Epithelial Cells
Infect. Immun., August 1, 2005; 73(8): 4653 - 4667.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Yuste, M. Botto, J. C. Paton, D. W. Holden, and J. S. Brown
Additive Inhibition of Complement Deposition by Pneumolysin and PspA Facilitates Streptococcus pneumoniae Septicemia
J. Immunol., August 1, 2005; 175(3): 1813 - 1819.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. J. Standish, U. H. Stroeher, and J. C. Paton
The two-component signal transduction system RR06/HK06 regulates expression of cbpA in Streptococcus pneumoniae
PNAS, May 24, 2005; 102(21): 7701 - 7706.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
A. M. Smith, C. Feldman, O. Massidda, K. McCarthy, D. Ndiweni, and K. P. Klugman
Altered PBP 2A and Its Role in the Development of Penicillin, Cefotaxime, and Ceftriaxone Resistance in a Clinical Isolate of Streptococcus pneumoniae
Antimicrob. Agents Chemother., May 1, 2005; 49(5): 2002 - 2007.
[Abstract] [Full Text] [PDF]

Home page
 Innate ImmunityHome page
M. W. Hornef and C. Bogdan
The role of epithelial Toll-like receptor expression in host defense and microbial tolerance
Innate Immunity, April 1, 2005; 11(2): 124 - 128.
[Abstract] [PDF]

Home page
 Infect. Immun.Home page
R. Palaniappan, S. Singh, U. P. Singh, S. K. K. Sakthivel, E. W. Ades, D. E. Briles, S. K. Hollingshead, J. C. Paton, J. S. Sampson, and J. W. Lillard Jr.
Differential PsaA-, PspA-, PspC-, and PdB-Specific Immune Responses in a Mouse Model of Pneumococcal Carriage
Infect. Immun., February 1, 2005; 73(2): 1006 - 1013.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. J. Orihuela, J. N. Radin, J. E. Sublett, G. Gao, D. Kaushal, and E. I. Tuomanen
Microarray Analysis of Pneumococcal Gene Expression during Invasive Disease
Infect. Immun., October 1, 2004; 72(10): 5582 - 5596.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
H.-Y. Kwon, A. D. Ogunniyi, M.-H. Choi, S.-N. Pyo, D.-K. Rhee, and J. C. Paton
The ClpP Protease of Streptococcus pneumoniae Modulates Virulence Gene Expression and Protects against Fatal Pneumococcal Challenge
Infect. Immun., October 1, 2004; 72(10): 5646 - 5653.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. S. Brown, S. M. Gilliland, S. Basavanna, and D. W. Holden
phgABC, a Three-Gene Operon Required for Growth of Streptococcus pneumoniae in Hyperosmotic Medium and In Vivo
Infect. Immun., August 1, 2004; 72(8): 4579 - 4588.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. Kadioglu, W. Coward, M. J. Colston, C. R. A. Hewitt, and P. W. Andrew
CD4-T-Lymphocyte Interactions with Pneumolysin and Pneumococci Suggest a Crucial Protective Role in the Host Response to Pneumococcal Infection
Infect. Immun., May 1, 2004; 72(5): 2689 - 2697.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
E. A. Joyce, A. Kawale, S. Censini, C. C. Kim, A. Covacci, and S. Falkow
LuxS Is Required for Persistent Pneumococcal Carriage and Expression of Virulence and Biosynthesis Genes
Infect. Immun., May 1, 2004; 72(5): 2964 - 2975.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
E. Swiatlo, J. King, G. S. Nabors, B. Mathews, and D. E. Briles
Pneumococcal Surface Protein A Is Expressed In Vivo, and Antibodies to PspA Are Effective for Therapy in a Murine Model of Pneumococcal Sepsis
Infect. Immun., December 1, 2003; 71(12): 7149 - 7153.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
H.-Y. Kwon, S.-W. Kim, M.-H. Choi, A. D. Ogunniyi, J. C. Paton, S.-H. Park, S.-N. Pyo, and D.-K. Rhee
Effect of Heat Shock and Mutations in ClpL and ClpP on Virulence Gene Expression in Streptococcus pneumoniae
Infect. Immun., July 1, 2003; 71(7): 3757 - 3765.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. Virtaneva, M. R. Graham, S. F. Porcella, N. P. Hoe, H. Su, E. A. Graviss, T. J. Gardner, J. E. Allison, W. J. Lemon, J. R. Bailey, et al.
Group A Streptococcus Gene Expression in Humans and Cynomolgus Macaques with Acute Pharyngitis
Infect. Immun., April 1, 2003; 71(4): 2199 - 2207.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2002 Society for General Microbiology.