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Prospecting for novel lipase genes using PCR^a

Department of Biological Sciences, Macquarie University, Sydney, New South Wales 2109, Australia¹
Physikalische Biochemie, Universitat Potsdam, Karl-Liebknecht-Strasse 24–25 D-14476 Golm, Germany²
Division of Molecular Medicine, University of Auckland School of Medicine, Private Bag 92109, Auckland, New Zealand³

Author for correspondence: Philip J. L. Bell. Tel: +61 2 98508157. Fax: +61 2 98508253. e-mail: pbell{at}rna.bio.mq.edu.au

A PCR method suitable for the isolation of lipase genes directly from environmental DNA is described. The problems associated with the low levels of similarity between lipase genes were overcome by extensive analysis of conserved regions and careful primer design. Using this method, a lipase gene (oli-lipase) was isolated directly from environmental DNA. This lipase showed less than 20% similarity with other known lipases at the amino acid level. The study also revealed that distantly related members of the /ß hydrolase superfamily share similar conserved motifs with the lipases, thus making these genes targets for gene prospecting by PCR.

Keywords: triacylglycerol hydrolase, biomass, thermophilic, environment

^a The GenBank accession number for the sequence reported in this paper is AF421484.

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