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Cross-talk between the L-sorbose and D-sorbitol (D-glucitol) metabolic pathways in Lactobacillus casei^a

Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Apdo. Correos 73, 46100 Burjassot, Spain¹

Author for correspondence: Gaspar Pérez-Martínez. Tel: +34 96 390 0022. Fax: +34 96 363 6301. e-mail: gaspar.perez{at}iata.csic.es

A gene encoding sorbitol-6-phosphate dehydrogenase (SorF) belonging to the sorbose operon (sorFABCDG) has been characterized in Lactobacillus casei. Inactivation of this gene revealed the presence of another sorbitol-6-phosphate dehydrogenase that was induced by D-sorbitol (D-glucitol). The gene encoding this activity (gutF) has also been isolated, sequenced and disrupted. The sorbitol-6-phosphate dehydrogenase genes (sorF, gutF) were required for growth on L-sorbose and D-sorbitol, respectively. Biochemical and transcriptional analyses of the wild-type and mutant strains demonstrated that L-sorbose and D-sorbitol induced sorF and the gene encoding the sorbose operon activator (sorR), while the expression of gutF was only activated by D-sorbitol. Furthermore, these studies indirectly suggested that a common metabolite of the L-sorbose and D-sorbitol metabolic pathways (probably D-sorbitol 6-phosphate) would act as the effector of SorR. The same effector would also be the inducer of gutF, although the two pathways seem to be subject to distinct regulatory mechanisms.

Keywords: sorbitol-6-phosphate dehydrogenase, sugar catabolism, gene regulation

Abbreviations: PTS, phosphotransferase system; Sor-PR, L-sorbose-1-phosphate reductase; Stol-PDh, D-sorbitol-6-phosphate dehydrogenase

^a The GenBank accession number for the sequence reported in this paper is AF396831.

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