| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Research Paper |
Laboratory of Microbial Interactions, Department of Immunology, Parasitology and Ultrastructure, Flanders Interuniversity Institute of Biotechnology, Vrije Universiteit Brussel, Paardenstraat 65, B-1640 Sint Genesius Rode, Belgium1
Author for correspondence: Pierre Cornelis. Tel: +32 2 3590221. Fax: +32 2 3590399. e-mail: pcornel{at}vub.ac.be
Vanadium has an antibacterial activity against Pseudomonas aeruginosa, especially under conditions of iron limitation. Some degree of resistance to V is inducible by prior exposure to the metal. One mutant (VS1) with a higher sensitivity to V was obtained by transposon mutagenesis of P. aeruginosa PA 59.20, a clinical isolate. This mutant had an insertion in a non-coding region, upstream of a cluster of four genes. Three of them show similarities to genes corresponding to known P. aeruginosa antibiotic efflux systems, including an efflux protein, a membrane fusion protein and an outer-membrane porin. This cluster was named mexGHI-opmD. By allelic exchange, three mutants, ncr (for non-coding region), mexI and opmD were constructed in P. aeruginosa PAO1. Next to V sensitivity, the ncr, mexI and opmD mutants also showed reduced production of elastase, rhamnolipids, pyocyanine, pyoverdine and had reduced swarming motility, phenotypes that are known to be regulated by quorum sensing. All wild-type phenotypes, including growth in the presence of V, were restored by complementation with the complete cluster. The production of N-acyl-homoserine lactones (AHLs) was detected using the Chromobacter violaceum bioassay. Total extracts from the three mutants failed to induce the production of violacein by C. violaceum, although AHLs were detected by TLC and C. violaceum overlay. Violacein production was restored by complementation with mexGHI-opmD. The opmD mutant grew very slowly in LB or CAA medium, indicating that OpmD has an important physiological function for the cell. In conclusion, it is believed that the MexGHI-OpmD pump is probably involved in AHL homeostasis in P. aeruginosa.
Keywords: Mex-type efflux pump, quorum sensing, N-acyl-homoserine lactone production
Abbreviations: AHL, N-acyl-homoserine lactone; BHL, N-butanoyl-L-homoserine lactone; OdDHL, N-(3-oxododecanoyl)-L-homoserine lactone; Cm, chloramphenicol; Gm, gentamicin; Tc, tetracycline
This article has been cited by other articles:
|
|
 |
|
  J. Overhage, M. Bains, M. D. Brazas, and R. E. W. Hancock Swarming of Pseudomonas aeruginosa Is a Complex Adaptation Leading to Increased Production of Virulence Factors and Antibiotic Resistance J. Bacteriol., April 15, 2008; 190(8): 2671 - 2679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Patriquin, E. Banin, C. Gilmour, R. Tuchman, E. P. Greenberg, and K. Poole Influence of Quorum Sensing and Iron on Twitching Motility and Biofilm Formation in Pseudomonas aeruginosa J. Bacteriol., January 15, 2008; 190(2): 662 - 671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Y. Chan, H. S. Bian, T. M. C. Tan, M. E. Mattmann, G. D. Geske, J. Igarashi, T. Hatano, H. Suga, H. E. Blackwell, and K. L. Chua Control of Quorum Sensing by a Burkholderia pseudomallei Multidrug Efflux Pump J. Bacteriol., June 1, 2007; 189(11): 4320 - 4324. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Depardieu, I. Podglajen, R. Leclercq, E. Collatz, and P. Courvalin Modes and Modulations of Antibiotic Resistance Gene Expression Clin. Microbiol. Rev., January 1, 2007; 20(1): 79 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Lujan, A. J. Moyano, I. Segura, C. E. Argarana, and A. M. Smania Quorum-sensing-deficient (lasR) mutants emerge at high frequency from a Pseudomonas aeruginosa mutS strain Microbiology, January 1, 2007; 153(1): 225 - 237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Gonzalez and N. D. Keshavan Messing with Bacterial Quorum Sensing Microbiol. Mol. Biol. Rev., December 1, 2006; 70(4): 859 - 875. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Hocquet, P. Nordmann, F. El Garch, L. Cabanne, and P. Plesiat Involvement of the MexXY-OprM Efflux System in Emergence of Cefepime Resistance in Clinical Strains of Pseudomonas aeruginosa. Antimicrob. Agents Chemother., April 1, 2006; 50(4): 1347 - 1351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yang, C. R. Lopez, and E. L. Zechiedrich Quorum sensing and multidrug transporters in Escherichia coli PNAS, February 14, 2006; 103(7): 2386 - 2391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Southey-Pillig, D. G. Davies, and K. Sauer Characterization of Temporal Protein Production in Pseudomonas aeruginosa Biofilms J. Bacteriol., December 1, 2005; 187(23): 8114 - 8126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Palma, J. Zurita, J. A. Ferreras, S. Worgall, D. H. Larone, L. Shi, F. Campagne, and L. E. N. Quadri Pseudomonas aeruginosa SoxR Does Not Conform to the Archetypal Paradigm for SoxR-Dependent Regulation of the Bacterial Oxidative Stress Adaptive Response Infect. Immun., May 1, 2005; 73(5): 2958 - 2966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Purevdorj-Gage, W. J. Costerton, and P. Stoodley Phenotypic differentiation and seeding dispersal in non-mucoid and mucoid Pseudomonas aeruginosa biofilms Microbiology, May 1, 2005; 151(5): 1569 - 1576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Sobel, S. Neshat, and K. Poole Mutations in PA2491 (mexS) Promote MexT-Dependent mexEF-oprN Expression and Multidrug Resistance in a Clinical Strain of Pseudomonas aeruginosa J. Bacteriol., February 15, 2005; 187(4): 1246 - 1253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Linares, J. A. Lopez, E. Camafeita, J. P. Albar, F. Rojo, and J. L. Martinez Overexpression of the Multidrug Efflux Pumps MexCD-OprJ and MexEF-OprN Is Associated with a Reduction of Type III Secretion in Pseudomonas aeruginosa J. Bacteriol., February 15, 2005; 187(4): 1384 - 1391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Llanes, D. Hocquet, C. Vogne, D. Benali-Baitich, C. Neuwirth, and P. Plesiat Clinical Strains of Pseudomonas aeruginosa Overproducing MexAB-OprM and MexXY Efflux Pumps Simultaneously Antimicrob. Agents Chemother., May 1, 2004; 48(5): 1797 - 1802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Smania, I. Segura, R. J. Pezza, C. Becerra, I. Albesa, and C. E. Argarana Emergence of phenotypic variants upon mismatch repair disruption in Pseudomonas aeruginosa Microbiology, May 1, 2004; 150(5): 1327 - 1338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Middlemiss and K. Poole Differential Impact of MexB Mutations on Substrate Selectivity of the MexAB-OprM Multidrug Efflux Pump of Pseudomonas aeruginosa J. Bacteriol., March 1, 2004; 186(5): 1258 - 1269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Li, T. Mima, Y. Komori, Y. Morita, T. Kuroda, T. Mizushima, and T. Tsuchiya A new member of the tripartite multidrug efflux pumps, MexVW-OprM, in Pseudomonas aeruginosa J. Antimicrob. Chemother., October 1, 2003; 52(4): 572 - 575. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sekiya, T. Mima, Y. Morita, T. Kuroda, T. Mizushima, and T. Tsuchiya Functional Cloning and Characterization of a Multidrug Efflux Pump, MexHI-OpmD, from a Pseudomonas aeruginosa Mutant Antimicrob. Agents Chemother., September 1, 2003; 47(9): 2990 - 2992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Vasil DNA Microarrays in Analysis of Quorum Sensing: Strengths and Limitations J. Bacteriol., April 1, 2003; 185(7): 2061 - 2065. [Full Text] [PDF]
|
|
|
|
|
|
V. E. Wagner, D. Bushnell, L. Passador, A. I. Brooks, and B. H. Iglewski Microarray Analysis of Pseudomonas aeruginosa Quorum-Sensing Regulons: Effects of Growth Phase and Environment J. Bacteriol., April 1, 2003; 185(7): 2080 - 2095. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
