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Microbiology (2002), 148, 2489-2495.
© 2002 Society for General Microbiology

Research Paper

Differential effects of Kid toxin on two modes of replication of lambdoid plasmids suggest that this toxin acts before, but not after, the assembly of the replication complex

Katarzyna Potrykus1, Sandra Santos2, Marc Lemonnier2, Ramon Diaz-Orejas2 and Grzegorz Wegrzyn1,3

Department of Molecular Biology, University of Gdask, Kladki 24, 80-822 Gdask, Poland1
Centro de Investigaciones Biológicas, C.S.I.C., Velázquez 144, 28006 Madrid, Spain2
Institute of Oceanology, Polish Academy of Sciences, w. Wojciecha 5, 81-347 Gdynia, Poland3

Author for correspondence: Grzegorz Wgrzyn. Tel: +48 58 346 3014. Fax: +48 58 301 0072. e-mail: wegrzyn{at}biotech.univ.gda.pl

Kid is a small protein that is encoded by plasmid R1. It is a toxin that belongs to a killer system that ensures the stability of the plasmid in host cells. The results of previous studies have suggested that Kid is an inhibitor of DNA replication, possibly acting at the onset of initiation. Here, the authors tested the effects of Kid on ori{lambda}-intitiated and oriJ-initiated replication, which may be driven by both the newly assembled replication complex and the heritable complex. It was found that Kid inhibits only replication that is driven by the newly assembled replication complex. The authors also report that Kid inhibits ColE1-like plasmid replication in vivo, in agreement with the previously reported inhibition of ColE1 during in vitro replication. It is proposed that the Kid toxin acts at the level of replication either by preventing de novo assembly of the replication complex or by impairing the functional interactions of the replication complex at the initiation stage.

Keywords: plasmid R1, {lambda} plasmids, oriJ-based plasmid, inheritance of the replication complex




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