| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Research Paper |
Molecular and Cellular Biology Program, Division of Infectious Diseases1 and Comparative Medicine Program, Department of Medicine,2 University of Maryland, Baltimore, School of Medicine, Baltimore, MD 21201, USA
Institut für Mikrobiologie und Genetik, Georg-August Universität, Grisebachstrasse 8, 37077 Göttingen, Germany3
Author for correspondence: Michael S. Donnenberg. Tel: +1 410 706 7560. Fax: +1 410 706 8700. e-mail: mdonnenb{at}umaryland.edu
A cluster of 14 genes located on the large plasmid of enteropathogenic Escherichia coli (EPEC) strains is sufficient to direct the biogenesis of the type IV bundle-forming pilus (BFP) in a recombinant E. coli host. The fifth gene in the cluster, bfpU, encodes a protein that is predicted to be localized to the periplasmic space. To determine whether BfpU is necessary for pilus biogenesis, the authors constructed a non-polar bfpU mutant EPEC strain by allelic exchange. The mutant strain was unable to perform localized adherence and auto-aggregation, two phenotypes associated with BFP expression, and it failed to make BFP. These phenotypes were restored to the bfpU mutant by a plasmid containing bfpU. There was no difference between the wild-type and bfpU mutant strains in their expression or processing of the pre-pilin protein or in their localization of the pilin protein in the inner and outer membranes. Fractionation studies revealed that BfpU is completely soluble and is detected in both the periplasm and the cytoplasm. Thus, BfpU represents a novel protein required for type IV pilus assembly.
Keywords: type IV fimbriae, periplasm, bundle-forming pili, bundlin
Abbreviations: BFP, bundle-forming pilus/pili; EPEC, enteropathogenic Escherichia coli
a These authors contributed equally to this paper.
This article has been cited by other articles:
|
|
 |
|
  S. J. Krebs, T. J. Kirn, and R. K. Taylor Genetic Mapping of Secretion and Functional Determinants of the Vibrio cholerae TcpF Colonization Factor J. Bacteriol., June 1, 2009; 191(11): 3665 - 3676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Daniel, A. Singh, L. J. Crowther, P. J. Fernandes, W. Schreiber, and M. S. Donnenberg Interaction and localization studies of enteropathogenic Escherichia coli type IV bundle-forming pilus outer membrane components. Microbiology, August 1, 2006; 152(Pt 8): 2405 - 2420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. De Windt, H. Gao, W. Kromer, P. Van Damme, J. Dick, J. Mast, N. Boon, J. Zhou, and W. Verstraete AggA is required for aggregation and increased biofilm formation of a hyper-aggregating mutant of Shewanella oneidensis MR-1. Microbiology, March 1, 2006; 152(Pt 3): 721 - 729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Z. Nevesinjac and T. L. Raivio The Cpx Envelope Stress Response Affects Expression of the Type IV Bundle-Forming Pili of Enteropathogenic Escherichia coli J. Bacteriol., January 15, 2005; 187(2): 672 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hwang, D. Bieber, S. W. Ramer, C.-Y. Wu, and G. K. Schoolnik Structural and Topographical Studies of the Type IV Bundle-Forming Pilus Assembly Complex of Enteropathogenic Escherichia coli J. Bacteriol., November 15, 2003; 185(22): 6695 - 6701. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Clarke, R. D. Haigh, P. P. E. Freestone, and P. H. Williams Virulence of Enteropathogenic Escherichia coli, a Global Pathogen Clin. Microbiol. Rev., July 1, 2003; 16(3): 365 - 378. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
