| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Research Paper |
Institut für Mikrobiologie, Heinrich-Heine-Universität, Universitätsstr. 1, Geb. 26.12.01, D-40225 Düsseldorf, Germany1
Department of Applied Microbiology, Lund University, 22100 Lund, Sweden2
Author for correspondence: Eckhard Boles. Tel: +49 211 81 12778. Fax: +49 211 81 15370. e-mail: boles{at}uni-duesseldorf.de
For an economically feasible production of ethanol from plant biomass by microbial cells, the fermentation of xylose is important. As xylose uptake might be a limiting step for xylose fermentation by recombinant xylose-utilizing Saccharomyces cerevisiae cells a study of xylose uptake was performed. After deletion of all of the 18 hexose-transporter genes, the ability of the cells to take up and to grow on xylose was lost. Reintroduction of individual hexose-transporter genes in this strain revealed that at intermediate xylose concentrations the yeast high- and intermediate-affinity transporters Hxt4, Hxt5, Hxt7 and Gal2 are important xylose-transporting proteins. Several heterologous monosaccharide transporters from bacteria and plant cells did not confer sufficient uptake activity to restore growth on xylose. Overexpression of the xylose-transporting proteins in a xylose-utilizing PUA yeast strain did not result in faster growth on xylose under aerobic conditions nor did it enhance the xylose fermentation rate under anaerobic conditions. The results of this study suggest that xylose uptake does not determine the xylose flux under the conditions and in the yeast strains investigated.
Keywords: Saccharomyces cerevisiae, xylose uptake, glucose uptake, xylose fermentation, heterologous expression
This article has been cited by other articles:
|
|
 |
|
  M. J. Leandro, I. Spencer-Martins, and P. Goncalves The expression in Saccharomyces cerevisiae of a glucose/xylose symporter from Candida intermedia is affected by the presence of a glucose/xylose facilitator Microbiology, June 1, 2008; 154(6): 1646 - 1655. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Wiedemann and E. Boles Codon-Optimized Bacterial Genes Improve L-Arabinose Fermentation in Recombinant Saccharomyces cerevisiae Appl. Envir. Microbiol., April 1, 2008; 74(7): 2043 - 2050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Guillaume, P. Delobel, J.-M. Sablayrolles, and B. Blondin Molecular Basis of Fructose Utilization by the Wine Yeast Saccharomyces cerevisiae: a Mutated HXT3 Allele Enhances Fructose Fermentation Appl. Envir. Microbiol., April 15, 2007; 73(8): 2432 - 2439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kawaguchi, A. A. Vertes, S. Okino, M. Inui, and H. Yukawa Engineering of a Xylose Metabolic Pathway in Corynebacterium glutamicum. Appl. Envir. Microbiol., May 1, 2006; 72(5): 3418 - 3428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-S. Jin, J. M. Laplaza, and T. W. Jeffries Saccharomyces cerevisiae Engineered for Xylose Metabolism Exhibits a Respiratory Response Appl. Envir. Microbiol., November 1, 2004; 70(11): 6816 - 6825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Liu, E. Boles, and B. P. Rosen Arsenic Trioxide Uptake by Hexose Permeases in Saccharomyces cerevisiae J. Biol. Chem., April 23, 2004; 279(17): 17312 - 17318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sonderegger, M. Jeppsson, B. Hahn-Hagerdal, and U. Sauer Molecular Basis for Anaerobic Growth of Saccharomyces cerevisiae on Xylose, Investigated by Global Gene Expression and Metabolic Flux Analysis Appl. Envir. Microbiol., April 1, 2004; 70(4): 2307 - 2317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Becker and E. Boles A Modified Saccharomyces cerevisiae Strain That Consumes L-Arabinose and Produces Ethanol Appl. Envir. Microbiol., July 1, 2003; 69(7): 4144 - 4150. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
