Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 148 (2002), 2903-2909
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nishimura, H.
Right arrow Articles by Kondo, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nishimura, H.
Right arrow Articles by Kondo, T.
Agricola
Right arrow Articles by Nishimura, H.
Right arrow Articles by Kondo, T.
Microbiology (2002), 148, 2903-2909.
© 2002 Society for General Microbiology

Research Paper

Mutations in KaiA, a clock protein, extend the period of circadian rhythm in the cyanobacterium Synechococcus elongatus PCC 7942

Hideya Nishimura1,2, Yoichi Nakahira1, Keiko Imai1, Akiko Tsuruhara1, Hisayo Kondo1, Hiroshi Hayashi2, Makoto Hirai2, Hidehiko Saito2 and Takao Kondo1

Division of Biological Science, Graduate School of Science, Nagoya University and CREST, Japan Science and Technology Corporation (JST), Furo-cho, Chikusa, Nagoya 464-8602, Japan1
First Department of Internal Medicine, Nagoya University, School of Medicine Tsurumai 65, Showa, Nagoya 466-8550, Japan2

Author for correspondence: Takao Kondo. Tel: +81 52 789 2498. Fax: +81 52 789 2963. e-mail: kondo{at}bio.nagoya-u.ac.jp

KaiA KaiB and KaiC are essential circadian clock proteins in the unicellular cyanobacterium Synechococcus elongatus PCC 7942. KaiA protein activates transcription of the kaiBC operon, which is believed to be a crucial step in the oscillating feedback loop of cyanobacteria. In this study, ~~400 mutations were introduced into kaiA by PCR-based mutagenesis, and rhythmic phenotypes of these mutants were studied by a bioluminescence reporter. In contrast to mutations in KaiB or KaiC, the vast majority of KaiA mutations extended the period and only rarely shortened it. The period could be extended to 35 h without lowering the mean or peak levels of kaiBC expression. However, several mutations resulted in low-amplitude oscillations or arrhythmia, which were accompanied by lowered kaiBC transcription. These results imply that the KaiA protein can change the period length of the circadian rhythm directly (through an unknown biochemical mechanism) or indirectly (by lowering kaiBC expression). Specific mutations of KaiA were identified in 34 mutants. While mutations mapped to various locations of the KaiA sequence, two clusters of period-altering mutations were found. This suggested that these regions are important domains of the KaiA protein for defining the period length. On the other hand, different sequences within KaiA to which arrhythmic mutations were mapped are important to enhance kaiBC expression.

Keywords: circadian clock, kai genes, kaiBC expression, amplitude of rhythm, bioluminescence rhythm

Abbreviations: LL, constant illumination; PMT, photomultiplier tube

These authors contributed equally to this work.




This article has been cited by other articles:


Home page
 Genes Dev.Home page
Y. Kitayama, T. Nishiwaki, K. Terauchi, and T. Kondo
Dual KaiC-based oscillations constitute the circadian system of cyanobacteria
Genes & Dev., June 1, 2008; 22(11): 1513 - 1521.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. Kutsuna, T. Kondo, H. Ikegami, T. Uzumaki, M. Katayama, and M. Ishiura
The Circadian Clock-Related Gene pex Regulates a Negative cis Element in the kaiA Promoter Region
J. Bacteriol., November 1, 2007; 189(21): 7690 - 7696.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
Y. Taniguchi, M. Katayama, R. Ito, N. Takai, T. Kondo, and T. Oyama
labA: a novel gene required for negative feedback regulation of the cyanobacterial circadian clock protein KaiC
Genes & Dev., January 1, 2007; 21(1): 60 - 70.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Takai, M. Nakajima, T. Oyama, R. Kito, C. Sugita, M. Sugita, T. Kondo, and H. Iwasaki
From the Cover: A KaiC-associating SasA-RpaA two-component regulatory system as a major circadian timing mediator in cyanobacteria
PNAS, August 8, 2006; 103(32): 12109 - 12114.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Iwase, K. Imada, F. Hayashi, T. Uzumaki, M. Morishita, K. Onai, Y. Furukawa, K. Namba, and M. Ishiura
Functionally Important Substructures of Circadian Clock Protein KaiB in a Unique Tetramer Complex
J. Biol. Chem., December 30, 2005; 280(52): 43141 - 43149.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Hitomi, T. Oyama, S. Han, A. S. Arvai, and E. D. Getzoff
Tetrameric Architecture of the Circadian Clock Protein KaiB: A NOVEL INTERFACE FOR INTERMOLECULAR INTERACTIONS AND ITS IMPACT ON THE CIRCADIAN RHYTHM
J. Biol. Chem., May 13, 2005; 280(19): 19127 - 19135.
[Abstract] [Full Text] [PDF]

Home page
 J Biol RhythmsHome page
E. Tauber, K. S. Last, P. J.W. Olive, and C. P. Kyriacou
Clock Gene Evolution and Functional Divergence
J Biol Rhythms, October 1, 2004; 19(5): 445 - 458.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Nishiwaki, Y. Satomi, M. Nakajima, C. Lee, R. Kiyohara, H. Kageyama, Y. Kitayama, M. Temamoto, A. Yamaguchi, A. Hijikata, et al.
From the Cover: Role of KaiC phosphorylation in the circadian clock system of Synechococcus elongatus PCC 7942
PNAS, September 21, 2004; 101(38): 13927 - 13932.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Imai, T. Nishiwaki, T. Kondo, and H. Iwasaki
Circadian Rhythms in the Synthesis and Degradation of a Master Clock Protein KaiC in Cyanobacteria
J. Biol. Chem., August 27, 2004; 279(35): 36534 - 36539.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
I. Vakonakis and A. C. LiWang
Structure of the C-terminal domain of the clock protein KaiA in complex with a KaiC-derived peptide: Implications for KaiC regulation
PNAS, July 27, 2004; 101(30): 10925 - 10930.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Ye, I. Vakonakis, T. R. Ioerger, A. C. LiWang, and J. C. Sacchettini
Crystal Structure of Circadian Clock Protein KaiA from Synechococcus elongatus
J. Biol. Chem., May 7, 2004; 279(19): 20511 - 20518.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
I. Vakonakis, J. Sun, T. Wu, A. Holzenburg, S. S. Golden, and A. C. LiWang
NMR structure of the KaiC-interacting C-terminal domain of KaiA, a circadian clock protein: Implications for KaiA-KaiC interaction
PNAS, February 10, 2004; 101(6): 1479 - 1484.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Nakahira, M. Katayama, H. Miyashita, S. Kutsuna, H. Iwasaki, T. Oyama, and T. Kondo
Global gene repression by KaiC as a master process of prokaryotic circadian system
PNAS, January 20, 2004; 101(3): 881 - 885.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. B. Williams, I. Vakonakis, S. S. Golden, and A. C. LiWang
Structure and function from the circadian clock protein KaiA of Synechococcus elongatus: A potential clock input mechanism
PNAS, November 26, 2002; 99(24): 15357 - 15362.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Iwasaki, T. Nishiwaki, Y. Kitayama, M. Nakajima, and T. Kondo
KaiA-stimulated KaiC phosphorylation in circadian timing loops in cyanobacteria
PNAS, November 26, 2002; 99(24): 15788 - 15793.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2002 Society for General Microbiology.