| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1682, USA
Correspondence
Julia M. Inamine
jinamine{at}colostate.edu
Members of the Mycobacterium avium complex are distinguished by the presence of highly antigenic surface molecules called glycopeptidolipids (GPLs) and the oligosaccharide portion of the serovar-specific GPL defines the 28 serovars. Previously, the genomic region (ser2) encoding the enzymes responsible for the glycosylation of the lipopeptide core to generate the serovar-2-specific GPLs has been described. In this work, the ser2 gene clusters of M. avium serovar 2 strains 2151 and TMC 724 were fully sequenced and compared to the homologous regions of M. avium serovar 1 strain 104, M. avium subsp. paratuberculosis and M. avium subsp. silvaticum. It was also determined that 104Rg, a mutant of strain 104 that produces truncated GPLs, lost several GPL biosynthesis genes by deletion. This comparison, together with analysis of protein similarities, supports a biosynthetic model in which serovar-2-specific GPLs are synthesized from a serovar-1-specific GPL intermediate that is derived from a non-specific GPL precursor. We also identified a gene encoding an enzyme that is necessary for the biosynthesis of serovar-3- and 9-specific GPLs, but not serovar-2-specific GPLs, suggesting that the different serovars may have evolved from the acquisition or loss of genetic information. In addition, a subcluster of genes for the biosynthesis and transfer of fucose, which are needed to make serovar-specific GPLs such as those of serovar 2, is found in the non-GPL-producing M. avium subspecies paratuberculosis and silvaticum.
The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are AF125999 and AF143772.
This article has been cited by other articles:
|
|
 |
|
  N. Fujiwara, N. Nakata, T. Naka, I. Yano, M. Doe, D. Chatterjee, M. McNeil, P. J. Brennan, K. Kobayashi, M. Makino, et al. Structural Analysis and Biosynthesis Gene Cluster of an Antigenic Glycopeptidolipid from Mycobacterium intracellulare J. Bacteriol., May 15, 2008; 190(10): 3613 - 3621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Nakata, N. Fujiwara, T. Naka, I. Yano, K. Kobayashi, and S. Maeda Identification and Characterization of Two Novel Methyltransferase Genes That Determine the Serotype 12-Specific Structure of Glycopeptidolipids of Mycobacterium intracellulare J. Bacteriol., February 1, 2008; 190(3): 1064 - 1071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Miyamoto, T. Mukai, Y. Maeda, N. Nakata, M. Kai, T. Naka, I. Yano, and M. Makino Characterization of the Fucosylation Pathway in the Biosynthesis of Glycopeptidolipids from Mycobacterium avium Complex J. Bacteriol., August 1, 2007; 189(15): 5515 - 5522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Y. Turenne, R. Wallace Jr., and M. A. Behr Mycobacterium avium in the Postgenomic Era Clin. Microbiol. Rev., April 1, 2007; 20(2): 205 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fujiwara, N. Nakata, S. Maeda, T. Naka, M. Doe, I. Yano, and K. Kobayashi Structural Characterization of a Specific Glycopeptidolipid Containing a Novel N-Acyl-Deoxy Sugar from Mycobacterium intracellulare Serotype 7 and Genetic Analysis of Its Glycosylation Pathway J. Bacteriol., February 1, 2007; 189(3): 1099 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Freeman, H. Geier, K. M. Weigel, J. Do, T. E. Ford, and G. A. Cangelosi Roles for Cell Wall Glycopeptidolipid in Surface Adherence and Planktonic Dispersal of Mycobacterium avium Appl. Envir. Microbiol., December 1, 2006; 72(12): 7554 - 7558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Miyamoto, T. Mukai, N. Nakata, Y. Maeda, M. Kai, T. Naka, I. Yano, and M. Makino Identification and Characterization of the Genes Involved in Glycosylation Pathways of Mycobacterial Glycopeptidolipid Biosynthesis J. Bacteriol., January 1, 2006; 188(1): 86 - 95. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Deshayes, F. Laval, H. Montrozier, M. Daffe, G. Etienne, and J.-M. Reyrat A Glycosyltransferase Involved in Biosynthesis of Triglycosylated Glycopeptidolipids in Mycobacterium smegmatis: Impact on Surface Properties J. Bacteriol., November 1, 2005; 187(21): 7283 - 7291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Blumenthal, J. Lauber, R. Hoffmann, M. Ernst, C. Keller, J. Buer, S. Ehlers, and N. Reiling Common and Unique Gene Expression Signatures of Human Macrophages in Response to Four Strains of Mycobacterium avium That Differ in Their Growth and Persistence Characteristics Infect. Immun., June 1, 2005; 73(6): 3330 - 3341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Domenech, M. B. Reed, and C. E. Barry III Contribution of the Mycobacterium tuberculosis MmpL Protein Family to Virulence and Drug Resistance Infect. Immun., June 1, 2005; 73(6): 3492 - 3501. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Burguiere, P. G. Hitchen, L. G. Dover, A. Dell, and G. S. Besra Altered expression profile of mycobacterial surface glycopeptidolipids following treatment with the antifungal azole inhibitors econazole and clotrimazole Microbiology, June 1, 2005; 151(6): 2087 - 2095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Krzywinska, J. Krzywinski, and J. S. Schorey Phylogeny of Mycobacterium avium strains inferred from glycopeptidolipid biosynthesis pathway genes Microbiology, June 1, 2004; 150(6): 1699 - 1706. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
