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Microbiology 149 (2003), 2901-2908; DOI  10.1099/mic.0.26435-0
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Microbiology 149 (2003), 2901-2908; DOI  10.1099/mic.0.26435-0
© 2003 Society for General Microbiology

Characterization of Enterococcus faecium mutants resistant to mundticin KS, a class IIa bacteriocin

Youko Sakayori1, Mizuho Muramatsu2, Satoshi Hanada2, Yoichi Kamagata2, Shinichi Kawamoto1 and Jun Shima1

1 National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan
2 Research Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan

Correspondence
Jun Shima
shimaj{at}nfri.affrc.go.jp

The emergence and spread of mutants resistant to bacteriocins would threaten the safety of using bacteriocins as food preservatives. To determine the physiological characteristics of resistant mutants, mutants of Enterococcus faecium resistant to mundticin KS, a class IIa bacteriocin, were isolated. Two types of mutant were found that had different sensitivities to other antimicrobial agents such as nisin (class I) and kanamycin. Both mutants were resistant to mundticin KS even in the absence of Mg2+ ions. The composition of unsaturated fatty acids in the resistant mutants was significantly increased in the presence of mundticin KS. The composition of the phospholipids in the two resistant mutants also differed from those in the wild-type strain. The putative zwitterionic amino-containing phospholipid in both mutants significantly increased, whereas amounts of phosphatidylglycerol and cardiolipin decreased. These changes in membrane structure may influence resistance of enterococci to class IIa and class I bacteriocins.

Abbreviations: ACP, amino-containing phospholipid; CL, cardiolipin; CPA, cyclopropanic acid; PG, phosphatidylglycerol






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