Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 149 (2003), 515-524; DOI  10.1099/mic.0.25959-0
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zverlov, V. V.
Right arrow Articles by Schwarz, W. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zverlov, V. V.
Right arrow Articles by Schwarz, W. H.
Agricola
Right arrow Articles by Zverlov, V. V.
Right arrow Articles by Schwarz, W. H.
Microbiology 149 (2003), 515-524; DOI  10.1099/mic.0.25959-0
© 2003 Society for General Microbiology

Two new cellulosome components encoded downstream of celI in the genome of Clostridium thermocellum: the non-processive endoglucanase CelN and the possibly structural protein CseP

Vladimir V. Zverlov1, Galina A. Velikodvorskaya1 and Wolfgang H. Schwarz2

1 Institute of Molecular Genetics, Russian Academy of Science, Kurchatov Sq., 123182 Moscow, Russia
2 Research Group Microbial Biotechnology, Technische Universität München, Am Hochanger 4, D-85350 Freising-Weihenstephan, Germany

Correspondence
Wolfgang H. Schwarz
schwarz{at}mikro.biologie.tu-muenchen.de

Clostridium thermocellum produces a great number of extracellular cellulases which are free or cellulosome-bound. The nucleotide sequence of a gene cluster containing the genes celI, celN and cseP was determined from C. thermocellum strain F7. Gene products Cel9I and Cel9N are structurally related enzymes having a glycosyl hydrolase family 9 and a carbohydrate-binding module (CBM3c), but show characteristic differences: Cel9I is a non-cellulosomal protein with an additional CBM (CBM3b), whereas Cel9N contains a cellulosomal dockerin module and no additional CBM. Although Cel9I is a processive endoglucanase, Cel9N is non-processive. Both enzymes hydrolyse phosphoric acid swollen cellulose, but the products of hydrolysis are different. The CseP protein encoded in the gene cluster is the first component attached to the cellulosomal scaffoldin for which no catalytic activity could be detected. It was shown to be present in the cellulosome. Its sequence is homologous to the spore-coat assembly protein CotH of Bacillus subtilis, suggesting a structural role of CseP in the cellulosome.

Abbreviations: CBM, carbohydrate-binding module; GHF, glycosyl hydrolase family; PASC, phosphoric acid swollen cellulose; pNP-, p-nitrophenyl-

The GenBank accession number for the sequence reported in this paper is AJ275974.




This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
V. V. Zverlov, M. Klupp, J. Krauss, and W. H. Schwarz
Mutations in the Scaffoldin Gene, cipA, of Clostridium thermocellum with Impaired Cellulosome Formation and Cellulose Hydrolysis: Insertions of a New Transposable Element, IS1447, and Implications for Cellulase Synergism on Crystalline Cellulose
J. Bacteriol., June 15, 2008; 190(12): 4321 - 4327.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
N. D. Gold and V. J. J. Martin
Global View of the Clostridium thermocellum Cellulosome Revealed by Quantitative Proteomic Analysis
J. Bacteriol., October 1, 2007; 189(19): 6787 - 6795.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
J.-C. Blouzard, C. Bourgeois, P. de Philip, O. Valette, A. Belaich, C. Tardif, J.-P. Belaich, and S. Pages
Enzyme Diversity of the Cellulolytic System Produced by Clostridium cellulolyticum Explored by Two-Dimensional Analysis: Identification of Seven Genes Encoding New Dockerin-Containing Proteins
J. Bacteriol., March 15, 2007; 189(6): 2300 - 2309.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
L. E. Taylor II, B. Henrissat, P. M. Coutinho, N. A. Ekborg, S. W. Hutcheson, and R. M. Weiner
Complete Cellulase System in the Marine Bacterium Saccharophagus degradans Strain 2-40T
J. Bacteriol., June 1, 2006; 188(11): 3849 - 3861.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. O. Han, H. Yukawa, M. Inui, and R. H. Doi
Molecular Cloning and Transcriptional and Expression Analysis of engO, Encoding a New Noncellulosomal Family 9 Enzyme, from Clostridium cellulovorans
J. Bacteriol., July 15, 2005; 187(14): 4884 - 4889.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y.-H. P. Zhang and L. R. Lynd
Cellulose utilization by Clostridium thermocellum: Bioenergetics and hydrolysis product assimilation
PNAS, May 17, 2005; 102(20): 7321 - 7325.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
A. L. Demain, M. Newcomb, and J. H. D. Wu
Cellulase, Clostridia, and Ethanol
Microbiol. Mol. Biol. Rev., March 1, 2005; 69(1): 124 - 154.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
H. Adelsberger, C. Hertel, E. Glawischnig, V. V. Zverlov, and W. H. Schwarz
Enzyme system of Clostridium stercorarium for hydrolysis of arabinoxylan: reconstitution of the in vivo system from recombinant enzymes
Microbiology, July 1, 2004; 150(7): 2257 - 2266.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2003 Society for General Microbiology.