HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Frehel, C. Articles by Charbit, A. Search for Related Content PubMed PubMed Citation Articles by Frehel, C. Articles by Charbit, A. Agricola Articles by Frehel, C. Articles by Charbit, A.

Capacity of ivanolysin O to replace listeriolysin O in phagosomal escape and in vivo survival of Listeria monocytogenes

Claude Frehel

Marie-Annick Lety

Laboratoire de Microbiologie, INSERM U-570, Faculté de Médecine Necker, 156 rue de Vaugirard, 75730 Paris Cedex 15, France

Correspondence
Alain Charbit
charbit{at}necker.fr

Listeriolysin O (LLO, hly-encoded) is a major virulence factor secreted by the pathogen Listeria monocytogenes. The amino acid sequence of LLO shows a high degree of similarity with that of ivanolysin O (ILO), the cytolysin secreted by the ruminant pathogen Listeria ivanovii. Here, it was tested whether ILO could functionally replace LLO by expressing the gene encoding ILO under the control of the hly promoter, in an hly-deleted strain of L. monocytogenes. It is shown that ILO allows efficient phagosomal escape of L. monocytogenes in both macrophages and hepatocytes. Moreover, expression of ILO is not cytotoxic and promotes normal intracellular multiplication. In vivo, the ILO-expressing strain can multiply and persist for several days in the liver of infected mice but is unable to survive in the spleen. This work underscores the key role played by the cytolysin in the virulence of pathogenic Listeria.

These authors contributed equally to this work.

This article has been cited by other articles:

				E. N. Bou Ghanem, D. S. McElroy, and S. E. F. D'Orazio Multiple Mechanisms Contribute to the Robust Rapid Gamma Interferon Response by CD8+ T Cells during Listeria monocytogenes Infection Infect. Immun., April 1, 2009; 77(4): 1492 - 1501. [Abstract] [Full Text] [PDF]

				H. Hara, I. Kawamura, T. Nomura, T. Tominaga, K. Tsuchiya, and M. Mitsuyama Cytolysin-Dependent Escape of the Bacterium from the Phagosome Is Required but Not Sufficient for Induction of the Th1 Immune Response against Listeria monocytogenes Infection: Distinct Role of Listeriolysin O Determined by Cytolysin Gene Replacement Infect. Immun., August 1, 2007; 75(8): 3791 - 3801. [Abstract] [Full Text] [PDF]

				T. Nomura, I. Kawamura, C. Kohda, H. Baba, Y. Ito, T. Kimoto, I. Watanabe, and M. Mitsuyama Irreversible loss of membrane-binding activity of Listeria-derived cytolysins in non-acidic conditions: a distinct difference from allied cytolysins produced by other Gram-positive bacteria Microbiology, July 1, 2007; 153(7): 2250 - 2258. [Abstract] [Full Text] [PDF]

				M.-A. Lety, C. Frehel, C. Raynaud, M. Dupuis, and A. Charbit Exploring the role of the CTL epitope region of listeriolysin O in the pathogenesis of Listeria monocytogenes. Microbiology, May 1, 2006; 152(Pt 5): 1287 - 1296. [Abstract] [Full Text] [PDF]

				D. W. Schuerch, E. M. Wilson-Kubalek, and R. K. Tweten Molecular basis of listeriolysin O pH dependence PNAS, August 30, 2005; 102(35): 12537 - 12542. [Abstract] [Full Text] [PDF]