| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik der Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstrasse 5, D-91058 Erlangen, Germany
Correspondence
Jörg Stülke
jstuelke{at}biologie.uni-erlangen.de
Glycolysis is one of the central routes of carbon catabolism in Bacillus subtilis. Several glycolytic enzymes, including the key enzyme glyceraldehyde-3-phosphate dehydrogenase, are encoded in the hexacistronic gapA operon. Expression of this operon is induced by a variety of sugars and amino acids. Under non-inducing conditions, expression is repressed by the CggR repressor protein, the product of the promoter-proximal gene of the operon. Here, it is shown that the amount of glyceraldehyde-3-phosphate dehydrogenase encoded by the second gene of the operon exceeds that of the CggR repressor by about 100-fold. This differential synthesis was attributed to an mRNA processing event that takes place at the 3' end of the cggR open reading frame and to differential segmental stabilities of the resulting cleavage products. The mRNA specifying the truncated cggR gene is quickly degraded, whereas the downstream processing products encompassing gapA are quite stable. This increased stability is conferred by the presence of a stem–loop structure at the 5' end of the processed mRNAs. Mutations were introduced in the region of the cleavage site. A mutation affecting the stability of the stem–loop structure immediately downstream of the processing site had two effects. First, the steady-state transcript pattern was drastically shifted towards the primary transcripts; second, the stability of the processed mRNA containing the destabilized stem–loop structure was strongly decreased. This results in a reduction of the amount of glyceraldehyde-3-phosphate dehydrogenase in the cell. It is concluded that mRNA processing is involved in differential syntheses of the proteins encoded by the gapA operon.
This article has been cited by other articles:
|
|
 |
|
  S. Zorrilla, T. Doan, C. Alfonso, E. Margeat, A. Ortega, G. Rivas, S. Aymerich, C. A. Royer, and N. Declerck Inducer-Modulated Cooperative Binding of the Tetrameric CggR Repressor to Operator DNA Biophys. J., May 1, 2007; 92(9): 3215 - 3227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Schilling, O. Frick, C. Herzberg, A. Ehrenreich, E. Heinzle, C. Wittmann, and J. Stulke Transcriptional and Metabolic Responses of Bacillus subtilis to the Availability of Organic Acids: Transcription Regulation Is Important but Not Sufficient To Account for Metabolic Adaptation Appl. Envir. Microbiol., January 1, 2007; 73(2): 499 - 507. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Maamar, L. Abdou, C. Boileau, O. Valette, and C. Tardif Transcriptional Analysis of the cip-cel Gene Cluster from Clostridium cellulolyticum. J. Bacteriol., April 1, 2006; 188(7): 2614 - 2624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Lorca, Y. J. Chung, R. D. Barabote, W. Weyler, C. H. Schilling, and M. H. Saier Jr Catabolite Repression and Activation in Bacillus subtilis: Dependency on CcpA, HPr, and HprK J. Bacteriol., November 15, 2005; 187(22): 7826 - 7839. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Gorke, E. Foulquier, and A. Galinier YvcK of Bacillus subtilis is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway Microbiology, November 1, 2005; 151(11): 3777 - 3791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Hames, S. Halbedel, O. Schilling, and J. Stulke Multiple-Mutation Reaction: a Method for Simultaneous Introduction of Multiple Mutations into the glpK Gene of Mycoplasma pneumoniae Appl. Envir. Microbiol., July 1, 2005; 71(7): 4097 - 4100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Even, O. Pellegrini, L. Zig, V. Labas, J. Vinh, D. Brechemmier-Baey, and H. Putzer Ribonucleases J1 and J2: two novel endoribonucleases in B.subtilis with functional homology to E.coli RNase E Nucleic Acids Res., April 14, 2005; 33(7): 2141 - 2152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Mader, S. Hennig, M. Hecker, and G. Homuth Transcriptional Organization and Posttranscriptional Regulation of the Bacillus subtilis Branched-Chain Amino Acid Biosynthesis Genes J. Bacteriol., April 15, 2004; 186(8): 2240 - 2252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Detsch and J. Stulke Ammonium utilization in Bacillus subtilis: transport and regulatory functions of NrgA and NrgB Microbiology, November 1, 2003; 149(11): 3289 - 3297. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
