HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Mora, D. Articles by Fortina, M. G. Search for Related Content PubMed PubMed Citation Articles by Mora, D. Articles by Fortina, M. G. Agricola Articles by Mora, D. Articles by Fortina, M. G.

16S–23S rRNA intergenic spacer region sequence variation in Streptococcus thermophilus and related dairy streptococci and development of a multiplex ITS-SSCP analysis for their identification

¹ Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, sezione Microbiologia Industriale, Università degli Studi di Milano, Milano, Italy
² Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, sezione Microbiologia Agraria, Alimentare e Ecologica, Università degli Studi di Milano, Milano, Italy

Correspondence
Diego Mora
diego.mora{at}unimi.it

The 16S–23S rRNA internal transcribed spacer (ITS) region of several Streptococcus thermophilus strains and some related dairy streptococci, S. macedonicus, S. salivarius and S. bovis, was analysed by sequence analysis. All the Streptococcus species were easily discriminated on the basis of sequence variations principally located upstream and downstream of the region encompassing the double-stranded processing sites and the tRNA^Ala gene. Comparison between tRNA^Ala gene sequences highlighted a high level of sequence conservation among the Streptococcus species investigated despite their belonging to separated phylogenetic clusters, i.e. the S. salivarius and S. bovis rRNA groups. A low but significant degree of variability was detected among the S. thermophilus strains, allowing the identification of four different ITS sequences. Similarity analysis of the ITS sequences showed that the Streptococcus species were clustered in two main branches, one containing S. macedonicus and S. bovis strains, and one containing S. thermophilus and S. salivarius strains. With the aim of developing a rapid tool for the identification of the dairy streptococci species a multiplex ITS-SSCP analysis of two discrete regions within the ITS locus was carried out.

The GenBank accession numbers for the sequences determined in this work are given in the text.

This article has been cited by other articles:

				M. Wang, B. Cao, Q. Yu, L. Liu, Q. Gao, L. Wang, and L. Feng Analysis of the 16S-23S rRNA Gene Internal Transcribed Spacer Region in Klebsiella Species J. Clin. Microbiol., November 1, 2008; 46(11): 3555 - 3563. [Abstract] [Full Text] [PDF]

				N. Sadeghifard, V. Gurtler, M. Beer, and R. J. Seviour The Mosaic Nature of Intergenic 16S-23S rRNA Spacer Regions Suggests rRNA Operon Copy Number Variation in Clostridium difficile Strains Appl. Envir. Microbiol., November 1, 2006; 72(11): 7311 - 7323. [Abstract] [Full Text] [PDF]

				M. Kumar and P. K. Shukla Use of PCR Targeting of Internal Transcribed Spacer Regions and Single-Stranded Conformation Polymorphism Analysis of Sequence Variation in Different Regions of rRNA Genes in Fungi for Rapid Diagnosis of Mycotic Keratitis J. Clin. Microbiol., February 1, 2005; 43(2): 662 - 668. [Abstract] [Full Text] [PDF]

				M. Shahamat, M. Alavi, J. E. M. Watts, J. M. Gonzalez, K. R. Sowers, D. W. Maeder, and F. T. Robb Development of Two PCR-Based Techniques for Detecting Helical and Coccoid Forms of Helicobacter pylori J. Clin. Microbiol., August 1, 2004; 42(8): 3613 - 3619. [Abstract] [Full Text] [PDF]

				M. Drancourt, V. Roux, P.-E. Fournier, and D. Raoult rpoB Gene Sequence-Based Identification of Aerobic Gram-Positive Cocci of the Genera Streptococcus, Enterococcus, Gemella, Abiotrophia, and Granulicatella J. Clin. Microbiol., February 1, 2004; 42(2): 497 - 504. [Abstract] [Full Text] [PDF]