| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Molecular Genetics and Biotechnology, The Hebrew University, Faculty of Medicine, POB 12272, Jerusalem 91120, Israel
2 Department of Biosciences, Teikyo University, 1-1 Toyosatodai, Utsunomiya 320-8551, Japan
Correspondence
Ilan Rosenshine
ilanro{at}cc.huji.ac.il
The flagellar apparatus consists of components that function as a type III secretion system (TTSS). Enteropathogenic and enterohaemorrhagic E. coli (EPEC and EHEC, respectively) produce an additional TTSS, which is involved in virulence via the translocation of effector proteins into infected host cells. This system is encoded by the locus of enterocyte effacement (LEE). The authors observed that EPEC and EHEC grown in Dulbecco's modified Eagle's medium to the mid- and late-exponential growth phase at 37 °C are non-motile. At the same time these conditions trigger the expression of the LEE-encoded TTSS. Furthermore, it was found that EPEC with an inactivated ihfA, which encodes the IHF
subunit of the integration host factor (IHF), becomes hyperflagellated and motile. Similar hypermotility was seen upon inactivation of the ihfA of EHEC strains. IHF-mediated repression of the EPEC flagella involves down-regulation of flhDC, which encodes a positive regulator of the flagellar regulon. IHF indirectly mediates flhDC repression, via a putative EPEC-unique regulator which is not encoded by LEE.
This article has been cited by other articles:
|
|
 |
|
  E. Stonehouse, G. Kovacikova, R. K. Taylor, and K. Skorupski Integration Host Factor Positively Regulates Virulence Gene Expression in Vibrio cholerae J. Bacteriol., July 1, 2008; 190(13): 4736 - 4748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Khan, S. Bouzari, C. Ma, C. M. Rosenberger, K. S. B. Bergstrom, D. L. Gibson, T. S. Steiner, and B. A. Vallance Flagellin-Dependent and -Independent Inflammatory Responses following Infection by Enteropathogenic Escherichia coli and Citrobacter rodentium Infect. Immun., April 1, 2008; 76(4): 1410 - 1422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Mellies, A. M. S. Barron, and A. M. Carmona Enteropathogenic and Enterohemorrhagic Escherichia coli Virulence Gene Regulation Infect. Immun., September 1, 2007; 75(9): 4199 - 4210. [Full Text] [PDF]
|
|
|
|
|
|
M. C. Lane, A. N. Simms, and H. L. T. Mobley Complex Interplay between Type 1 Fimbrial Expression and Flagellum-Mediated Motility of Uropathogenic Escherichia coli J. Bacteriol., August 1, 2007; 189(15): 5523 - 5533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Russell, F. C. Sharp, D. A. Rasko, and V. Sperandio QseA and GrlR/GrlA Regulation of the Locus of Enterocyte Effacement Genes in Enterohemorrhagic Escherichia coli J. Bacteriol., July 15, 2007; 189(14): 5387 - 5392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Iyoda, N. Koizumi, H. Satou, Y. Lu, T. Saitoh, M. Ohnishi, and H. Watanabe The GrlR-GrlA Regulatory System Coordinately Controls the Expression of Flagellar and LEE-Encoded Type III Protein Secretion Systems in Enterohemorrhagic Escherichia coli. J. Bacteriol., August 1, 2006; 188(16): 5682 - 5692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Nadler, Y. Shifrin, S. Nov, S. Kobi, and I. Rosenshine Characterization of Enteropathogenic Escherichia coli Mutants That Fail To Disrupt Host Cell Spreading and Attachment to Substratum Infect. Immun., February 1, 2006; 74(2): 839 - 849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Torres, X. Zhou, and J. B. Kaper Adherence of Diarrheagenic Escherichia coli Strains to Epithelial Cells Infect. Immun., January 1, 2005; 73(1): 18 - 29. [Full Text] [PDF]
|
|
|
|
|
|
T. Berdichevsky, D. Friedberg, C. Nadler, A. Rokney, A. Oppenheim, and I. Rosenshine Ler Is a Negative Autoregulator of the LEE1 Operon in Enteropathogenic Escherichia coli J. Bacteriol., January 1, 2005; 187(1): 349 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Li, I. Rosenshine, S. L. Tung, X. H. Wang, D. Friedberg, C. L. Hew, and K. Y. Leung Comparative Proteomic Analysis of Extracellular Proteins of Enterohemorrhagic and Enteropathogenic Escherichia coli Strains and Their ihf and ler Mutants Appl. Envir. Microbiol., September 1, 2004; 70(9): 5274 - 5282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Monday, S. A. Minnich, and P. C. H. Feng A 12-Base-Pair Deletion in the Flagellar Master Control Gene flhC Causes Nonmotility of the Pathogenic German Sorbitol-Fermenting Escherichia coli O157:H- Strains J. Bacteriol., April 15, 2004; 186(8): 2319 - 2327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Cleary, L.-C. Lai, R. K. Shaw, A. Straatman-Iwanowska, M. S. Donnenberg, G. Frankel, and S. Knutton Enteropathogenic Escherichia coli (EPEC) adhesion to intestinal epithelial cells: role of bundle-forming pili (BFP), EspA filaments and intimin Microbiology, March 1, 2004; 150(3): 527 - 538. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
