Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oh, J.-I.
Right arrow Articles by Kaplan, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oh, J.-I.
Right arrow Articles by Kaplan, S.
Agricola
Right arrow Articles by Oh, J.-I.
Right arrow Articles by Kaplan, S.
Microbiology 149 (2003), 949-960; DOI  10.1099/mic.0.26010-0
© 2003 Society for General Microbiology

Digging deeper: uncovering genetic loci which modulate photosynthesis gene expression in Rhodobacter sphaeroides 2.4.1

Jeong-II Oh{dagger}, In-Jeong Ko and Samuel Kaplan

Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center, Medical School, 6431 Fannin, Houston, TX 77030, USA

Correspondence
Samuel Kaplan
samuel.kaplan{at}uth.tmc.edu

A new genetic locus was identified in Rhodobacter sphaeroides which is required for optimal synthesis of the light-harvesting spectral complexes as well as for optimal growth under anaerobic conditions with dimethyl sulfoxide (DMSO) as a terminal electron acceptor. The primary structure of the deduced osp gene product shows significant homology to the receiver domain of known response regulators common to bacterial two-component systems. However, site-directed mutagenesis revealed that the Osp protein appears not to be involved in a phospho-relay signal transduction pathway. Paradoxically, the effect of the Osp protein upon spectral complex levels is exerted at the transcriptional level of photosynthesis gene expression. The absence of the Osp protein does not appear to have a general effect on house-keeping metabolism. In cells lacking Osp, the levels of DMSO reductase appear to be normal. The quaternary structure of the Osp protein was determined to be a homodimer and it was directly demonstrated that Osp does not bind to the promoter region of photosynthesis genes as judged by mobility-shift experiments and primary structure analysis.

Abbreviations: PS, photosynthesis

The GenBank accession number for the osp gene sequence reported in this article is AF547169.

{dagger}Present address: Department of Microbiology, Pusan National University, Pusan, Korea.




This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
H. Arai, J. H. Roh, and S. Kaplan
Transcriptome Dynamics during the Transition from Anaerobic Photosynthesis to Aerobic Respiration in Rhodobacter sphaeroides 2.4.1
J. Bacteriol., January 1, 2008; 190(1): 286 - 299.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
C. L. Tavano, A. M. Podevels, and T. J. Donohue
Identification of Genes Required for Recycling Reducing Power during Photosynthetic Growth
J. Bacteriol., August 1, 2005; 187(15): 5249 - 5258.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. H. Roh, W. E. Smith, and S. Kaplan
Effects of Oxygen and Light Intensity on Transcriptome Expression in Rhodobacter sphaeroides 2.4.1: REDOX ACTIVE GENE EXPRESSION PROFILE
J. Biol. Chem., March 5, 2004; 279(10): 9146 - 9155.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2003 Society for General Microbiology.