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Microbiology 149 (2003), 1183-1191; DOI  10.1099/mic.0.25993-0
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Microbiology 149 (2003), 1183-1191; DOI  10.1099/mic.0.25993-0
© 2003 Society for General Microbiology

Isolation and characterization of two specific regulatory Aspergillus niger mutants shows antagonistic regulation of arabinan and xylan metabolism

Marco J. L. de Groot{dagger}, Peter J. I. van de Vondervoort{ddagger}, Ronald P. de Vries§, Patricia A. vanKuyk||, George J. G. Ruijter and Jaap Visser#

Section Molecular Genetics of Industrial Micro-organisms, Wageningen University, Dreijenlaan 2, NL-6703HA Wageningen, The Netherlands

Correspondence
Marco J. L. de Groot
Marco.de.Groot{at}BPRC.nl

This paper describes two Aspergillus niger mutants (araA and araB) specifically disturbed in the regulation of the arabinanase system in response to the presence of L-arabinose. Expression of the three known L-arabinose-induced arabinanolytic genes, abfA, abfB and abnA, was substantially decreased or absent in the araA and araB strains compared to the wild-type when incubated in the presence of L-arabinose or L-arabitol. In addition, the intracellular activities of L-arabitol dehydrogenase and L-arabinose reductase, involved in L-arabinose catabolism, were decreased in the araA and araB strains. Finally, the data show that the gene encoding D-xylulose kinase, xkiA, is also under control of the arabinanolytic regulatory system. L-Arabitol, most likely the true inducer of the arabinanolytic and L-arabinose catabolic genes, accumulated to a high intracellular concentration in the araA and araB mutants. This indicates that the decrease of expression of the arabinanolytic genes was not due to lack of inducer accumulation. Therefore, it is proposed that the araA and araB mutations are localized in positive-acting components of the regulatory system involved in the expression of the arabinanase-encoding genes and the genes encoding the L-arabinose catabolic pathway.

{dagger}Present address: Biomedical Primate Research Centre, Department of Parasitology, PO Box 3306, NL-2280GH Rijswijk, The Netherlands.

{ddagger}Present address: Laboratory of Phytopathology, Wageningen University, Binnenhaven 5, NL-6709 PD Wageningen, The Netherlands.

§Present address: Microbiology, Utrecht University, Padualaan 8, NL-3584CH Utrecht, The Netherlands.

||Present address: Leiden University, Institute Biology Leiden, Clusius Laboratory, Dept of Fungal Genetics and Metabolomics, NL-2333 AL Leiden.

Present address: Department of Clinical Genetics, Leiden University Medical Centre, PO Box 9600, NL-2300RC Leiden, The Netherlands.

#Present address: Fungal Genetics and Technology Consultancy, PO Box 396, NL-6700AJ Wageningen, The Netherlands.






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