Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kuchanny-Ardigò, D.
Right arrow Articles by Lipinska, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kuchanny-Ardigò, D.
Right arrow Articles by Lipinska, B.
Agricola
Right arrow Articles by Kuchanny-Ardigò, D.
Right arrow Articles by Lipinska, B.
Microbiology 149 (2003), 1483-1492; DOI  10.1099/mic.0.26273-0
© 2003 Society for General Microbiology

Cloning and characterization of the groE heat-shock operon of the marine bacterium Vibrio harveyi

Dorota Kuchanny-Ardigò and Barbara Lipinska

Department of Biochemistry, University of Gdansk, Kladki 24, 80-822 Gdansk, Poland

Correspondence
Barbara Lipinska
lipinska{at}biotech.univ.gda.pl

The DNA region of the Vibrio harveyi chromosome containing the heat-shock genes groES and groEL was cloned, and the genes were sequenced. These genes are arranged in the chromosome in the order groES–groEL. Northern hybridization experiments with RNA from V. harveyi and a DNA probe carrying both groES and groEL genes showed a single, heat-inducible transcript of approximately 2200 nt, indicating that these genes form an operon. Primer extension analysis revealed a strong, heat-inducible transcription start site 59 nt upstream of groES, preceded by a sequence typical for the Escherichia coli heat-shock promoters recognized by the {sigma}32 factor, and a weak transcription start site 25 nt upstream the groES gene, preceded by a sequence typical for {sigma}70 promoters. Transcription from the latter promoter occurred only at low temperatures. The V. harveyi groE operon cloned in a plasmid in E. coli cells was transcribed in a {sigma}32-dependent manner; the transcript size and the {sigma}32-dependent transcription start site were as in V. harveyi cells. Comparison of V. harveyi groE transcription regulation with the other well-characterized groE operons of the {gamma} subdivision of proteobacteria (those of E. coli and Pseudomonas aeruginosa) indicates a high conservation of the transcriptional regulatory elements among these bacteria, with two promoters, {sigma}32 and {sigma}70, involved in the regulation. The ability of the cloned groESL genes to complement E. coli groE mutants was tested: V. harveyi groES restored a thermoresistant phenotype to groES bacteria and enabled {lambda} phage to grow in the mutant cells. V. harveyi groEL did not abolish thermosensitivity of groEL bacteria but it complemented the groEL mutant with respect to growth of {lambda} phage. The results suggest that the GroEL chaperone may be more species-specific than the GroES co-chaperone.

Abbreviations: CIRCE, controlling inverted repeat of chaperone expression; Ts, temperature sensitive

The GenBank accession number for the sequence reported in this paper is AY246431.




This article has been cited by other articles:


Home page
 Infect. Immun.Home page
Y. Du and C. G. Arvidson
RpoH Mediates the Expression of Some, but Not All, Genes Induced in Neisseria gonorrhoeae Adherent to Epithelial Cells.
Infect. Immun., May 1, 2006; 74(5): 2767 - 2776.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2003 Society for General Microbiology.