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Microbiology 149 (2003), 1503-1511; DOI  10.1099/mic.0.25827-0
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Microbiology 149 (2003), 1503-1511; DOI  10.1099/mic.0.25827-0
© 2003 Society for General Microbiology

Transposition in Lactobacillus delbrueckii subsp. bulgaricus: identification of two thermosensitive replicons and two functional insertion sequences

Pascale Serror1, Golnar Ilami2, Hichem Chouayekh2, S. Dusko Ehrlich2 and Emmanuelle Maguin2

1 Unité Recherche Laitière et Génétique Appliquée, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France
2 Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France

Correspondence
Pascale Serror
serror{at}jouy.inra.fr

In this report, it is shown that the rolling circle replicon pG+host and the theta replicon pIP501 are thermosensitive in Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus). Using a pIP501 derivative as a delivery vector for six insertion sequences originating from lactic acid bacteria, it is shown that IS1223 and IS1201 transpose in L. bulgaricus.

Abbreviations: IS, insertion sequence

The GenBank/EMBL/DDBJ accession number for the ISLre1 sequence is AF449484.






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