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Biomedical Research, The University of Texas Health Center at Tyler, 11937 US Hwy 271, Tyler, TX 75708, USA
Correspondence
Malini Rajagopalan
malini.rajagopalan{at}uthct.edu
To understand the role of Mycobacterium smegmatis ftsZ (ftsZsmeg) in the cell division process, the ftsZ gene was characterized at the genetic level. This study shows that ftsZsmeg is an essential gene in that it can only be disrupted in a merodiploid background carrying another functional copy. Expression of ftsZsmeg in M. smegmatis from a constitutively active mycobacterial promoter resulted in lethality whereas that from a chemically inducible acetamidase (ami) promoter led to FtsZ accumulation, filamentation and cell lysis. To further understand the roles of ftsZ in cell division a conditionally complementing ftsZsmeg mutant strain was constructed in which ftsZ expression is controlled by acetamide. Growth in the presence of 0·2 % acetamide increased FtsZ levels approximately 1·4-fold, but did not decrease viability or change cell length. Withdrawal of acetamide reduced FtsZ levels, decreased viability, increased cell length and eventually lysed the cells. Finally, it is shown that ftsZsmeg function in M. smegmatis can be replaced with the Mycobacterium tuberculosis counterpart, indicating that heterologous FtsZtb can independently initiate the formation of Z-rings and catalyse the septation process. It is concluded that optimal levels of M. smegmatis FtsZ are required to sustain cell division and that the cell division initiation mechanisms are similar in mycobacteria.
Present address: Centre for Microbiology & Virology, Polish Academy of Sciences, Lodowa 106, 93-231 
odz, Poland.
These authors contributed equally to the work.
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