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Microbiology 149 (2003), 1971-1979; DOI  10.1099/mic.0.26320-0
© 2003 Society for General Microbiology

Physiological role of S-formylglutathione hydrolase in C1 metabolism of the methylotrophic yeast Candida boidinii

Hiroya Yurimoto, Bumjun Lee, Taisuke Yano, Yasuyoshi Sakai and Nobuo Kato

Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan

Correspondence
Yasuyoshi Sakai
ysakai{at}kais.kyoto-u.ac.jp

The methylotrophic yeast Candida boidinii exhibits S-formylglutathione hydrolase activity (FGH, EC 3.1.2.12), which is involved in the glutathione-dependent formaldehyde oxidation pathway during growth on methanol as the sole carbon source. The structural gene, FGH1, was cloned from C. boidinii, and its predicted amino acid sequence showed more than 60 % similarity to those of FGHs from Paracoccus denitrificans and Saccharomyces cerevisiae, and human esterase D. FGH from C. boidinii contained a C-terminal tripeptide, SKL, which is a type I peroxisome-targeting signal, and a bimodal distribution of FGH between peroxisomes and the cytosol was demonstrated. The FGH1 gene was disrupted in the C. boidinii genome by one-step gene disruption. The fgh1{Delta} strain was still able to grow on methanol as a carbon source under methanol-limited chemostat conditions with low dilution rates (D<0·05 h-1), conditions under which a strain with disruption of the gene for formaldehyde dehydrogenase (another enzyme involved in the formaldehyde oxidation pathway) could not survive. These results suggested that FGH is not essential but necessary for optimal growth on methanol. This is believed to be the first report of detailed analyses of the FGH1 gene in a methylotrophic yeast strain.

Abbreviations: AOD, alcohol oxidase; FDH, formate dehydrogenase; FGH, S-formylglutathione hydrolase; FLD, glutathione-dependent formaldehyde dehydrogenase; GFP, green fluorescent protein; GSH, reduced form of glutathione; GS-SG, oxidized form of glutathione; PTS, peroxisome-targeting signal

The GenBank accession number for the sequence reported in this paper is AB104827.






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