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Effect of EDTA on Salmonella enterica serovar Typhimurium involves a component not assignable to lipopolysaccharide release

¹ VTT Biotechnology, PO Box 1500, FIN-02044 VTT, Espoo, Finland
² Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, PO Box 56, FIN-00014 University of Helsinki, Finland

Correspondence
H.-L. Alakomi
Hanna-Leena.Alakomi{at}vtt.fi

The effect of EDTA on Salmonella enterica serovar Typhimurium was studied in different growth phases with cells grown with or without Ca²⁺ and Mg²⁺ supplementation. EDTA affected the outer membrane much more strongly in the early exponential phase than in the mid- or late exponential phase, as indicated by uptake of 1-N-phenylnaphthylamine (a nonpolar hydrophobic probe, M_r 219), and detergent (SDS) susceptibility. This effect was, however, not paralleled by LPS release (determined by measuring LPS-specific fatty acids or ¹⁴C-labelled LPS in cell-free supernatants, per a standardized cell density), which remained unchanged as a function of the growth curve. The conclusion from these results is that in the early exponential phase the effect of EDTA in S. enterica involves a component that is independent of LPS release.

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