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Microbiology 149 (2003), 2251-2262; DOI  10.1099/mic.0.26270-0
© 2003 Society for General Microbiology

Nitrogen fixation genetics and regulation in a Pseudomonas stutzeri strain associated with rice

Nicole Desnoues1, Min Lin2, Xianwu Guo1,3,{dagger}, Luyan Ma1,{ddagger}, Ricardo Carreño-Lopez1,§ and Claudine Elmerich1,4

1 Microbiologie et Environnement, CNRS URA D2172, Institut Pasteur, Paris, France
2 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, PR China
3 Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
4 Institut des Sciences du Végétal, CNRS UPR 2355, Bâtiment 23, Avenue de la Terrasse, 91198 Gif sur Yvette, France

Correspondence
Claudine Elmerich
Claudine.Elmerich{at}isv.cnrs-gif.fr

The Pseudomonas stutzeri strain A1501 (formerly known as Alcaligenes faecalis) fixes nitrogen under microaerobic conditions in the free-living state and colonizes rice endophytically. The authors characterized a region in strain A1501, corresponding to most of the nif genes and the rnf genes, involved in electron transport to nitrogenase in Rhodobacter capsulatus. The region contained three groups of genes arranged in the same order as in Azotobacter vinelandii: (1) nifB fdx ORF3 nifQ ORF5 ORF6; (2) nifLA-rnfABCDGEF-nifY2/nafY; (3) ORF13 ORF12-nifHDK-nifTY ORF1 ORF2-nifEN. Unlike in A. vinelandii, where these genes are not contiguous on the chromosome, but broken into two regions of the genome, the genes characterized here in P. stutzeri are contiguous and present on a 30 kb region in the genome of this organism. Insertion mutagenesis confirmed that most of the nif and the rnf genes in A1501 were essential for nitrogen fixation. Using lacZ fusions it was found that nif and rnf gene expression was under the control of ntrBC, nifLA and rpoN and that the rnf gene products were involved in the regulation of the nitrogen fixation process.

The GenBank accession numbers for the sequences reported in this paper are AJ297529.2; AJ313205 and AJ320536.

{dagger}Permanent address: Institute of Life Science and Technology, Huazhong Agricultural University, PR China.

{ddagger}Permanent address: National Laboratories for Agrobiotechnology, Beijing Agricultural University, Beijing 100094, PR China.

§Present addresss: Instituto de Ciencias, Universidad Autónoma de Puebla, CP 72000, Puebla, Pue, Mexico.




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