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1 Institut für Hygiene und Mikrobiologie der Bayerischen Julius-Maximilians-Universität, 97080 Würzburg, Germany
2 Institut für Hygiene, 48149 Münster, Germany
3 Poliklinik für Parodontologie der Westfälischen Wilhelms-Universität, 48149 Münster, Germany
Correspondence
Herbert Schmidt
Herbert.Schmidt{at}mailbox.tu-dresden.de
In this study, the genetic relationship of 19 Porphyromonas gingivalis isolates from patients with periodontitis was investigated by multilocus sequence analysis. Internal 400–600 bp DNA fragments of the 10 chromosomal genes ef-tu, ftsQ, hagB, gpdxJ, pepO, mcmA, dnaK, recA, pga and nah were amplified by PCR and sequenced. No two isolates were identical at all 10 loci. Phylogenetic analyses indicated a panmictic population structure of P. gingivalis. Split decomposition analysis, calculation of homoplasy ratios and analyses of clustered polymorphisms all indicate that recombination plays a major role in creating the genetic heterogeneity of P. gingivalis. A standardized index of association of 0·0898 indicates that the P. gingivalis genes analysed are close to linkage equilibrium.
, standardized index of associationThe nucleotide sequences of the fragments of all Porphyromonas gingivalis genes and their alleles (190 sequences) reported in this paper have been entered into the EMBL/GenBank databases and have received continuous accession numbers from AJ555632 to AJ555821.
Present address: Institut für Medizinische Mikrobiologie und Krankenhaushygiene, Medizinische Hochschule Hannover, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.
Present address: Institut für Medizinische Mikrobiologie und Hygiene, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
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