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Chlamydia trachomatis ²⁸ recognizes the fliC promoter of Escherichia coli and responds to heat shock in chlamydiae

Section of Infectious Diseases, Department of Medicine, Boston Medical Center, Boston University School of Medicine, Boston, Massachusetts 02118, USA

Correspondence
You-xun Zhang
yxzhang{at}bu.edu

The rpsD gene of Chlamydia trachomatis encodes the alternative factor ²⁸, which bears strong homology to many bacterial factors, including Escherichia coli ²⁸ and Bacillus subtilis ^B and ^D. Recently, a ²⁸ promoter was identified upstream of the late-cycle-expressed gene hctB, which encodes the Chlamydia-histone-like protein 2 (Yu & Tan, 2003). In this study it is shown that the product of chlamydial rpsD is an E. coli ²⁸ homologue. It was found that recombinant chlamydial ²⁸, in combination with E. coli core RNA polymerase, initiates transcription in vitro from the E. coli ²⁸-dependent promoter of fliC. It was also demonstrated that the recombinant chlamydial ²⁸ does not recognize major factor ⁷⁰-consensus-like sequences in vitro. In C. trachomatis-infected cells, two rpsD transcripts were detected with 5' ends located 18 (transcript I) and 54 bp (transcript II) upstream of the translational initiation codon at 16 and 30 h post-infection. When the temperature of cultures infected with C. trachomatis was shifted from 35 to 42 °C, the rpsD transcript I increased dramatically. The levels of chlamydial ²⁸, relative to EF-Tu, were greater throughout the exponential growth phase of the reticulate body, but lower late in the developmental cycle. These data support the hypothesis that ²⁸ plays a role in the regulatory network that allows chlamydiae to survive changes in its environment, enabling it to complete its unique developmental cycle.

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