| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Naval Medical Research Center, Silver Spring, MD 20910, USA
2 BBSRC Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney Lane, Colney, Norwich NR4 7UA, UK
Correspondence
Jerry M. Wells
Jerry.Wells{at}bbsrc.ac.uk
Two large tetracycline resistance (TcR) plasmids have been completely sequenced, the pTet plasmid (45·2 kb) from Campylobacter jejuni strain 81-176 and a plasmid pCC31 (44·7 kb) from Campylobacter coli strain CC31 that was isolated from a human case of severe gastroenteritis in the UK. Both plasmids are mosaic in structure, having homologues of genes found in a variety of different commensal and pathogenic bacteria, but nevertheless, showed striking similarities in DNA sequence and overall gene organization. Several predicted proteins encoded by genes involved in conjugation showed highest homology to proteins found in Actinobacillus actinomycetemcomitans, a periodontal pathogen. In addition to replication- and conjugation-associated genes, both plasmids carried a tet(O) gene encoding tetracycline resistance, a 6 kb ORF encoding a putative methylase and a number of genes of unknown function. The pTet plasmid co-exists in C. jejuni strain 81-176 with a smaller, previously characterized, non-conjugative plasmid pVir that also encodes a type IV secretion system (T4SS) that may affect virulence. In contrast, the T4SS encoded by pTet and pCC31 are shown to mediate bacterial conjugation between Campylobacter. The possible origin and evolution of pCC31 and pTet is discussed.
The sequence of the pTet and pCC31 plasmids have been deposited in GenBank under accession numbers AY394561 and AY394560, respectively.
This article has been cited by other articles:
|
|
 |
|
  C. Romo-Gonzalez, N. R. Salama, J. Burgeno-Ferreira, V. Ponce-Castaneda, E. Lazcano-Ponce, M. Camorlinga-Ponce, and J. Torres Differences in Genome Content among Helicobacter pylori Isolates from Patients with Gastritis, Duodenal Ulcer, or Gastric Cancer Reveal Novel Disease-Associated Genes Infect. Immun., May 1, 2009; 77(5): 2201 - 2211. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Chee-Sanford, R. I. Mackie, S. Koike, I. G. Krapac, Y.-F. Lin, A. C. Yannarell, S. Maxwell, and R. I. Aminov Fate and Transport of Antibiotic Residues and Antibiotic Resistance Genes following Land Application of Manure Waste J. Environ. Qual., April 27, 2009; 38(3): 1086 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Poly, T. D. Read, Y.-H. Chen, M. A. Monteiro, O. Serichantalergs, P. Pootong, L. Bodhidatta, C. J. Mason, D. Rockabrand, S. Baqar, et al. Characterization of Two Campylobacter jejuni Strains for Use in Volunteer Experimental-Infection Studies Infect. Immun., December 1, 2008; 76(12): 5655 - 5667. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Arechaga, A. Pena, S. Zunzunegui, M. del Carmen Fernandez-Alonso, G. Rivas, and F. de la Cruz ATPase Activity and Oligomeric State of TrwK, the VirB4 Homologue of the Plasmid R388 Type IV Secretion System J. Bacteriol., August 1, 2008; 190(15): 5472 - 5479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yun, B. Jeon, Y.-W. Barton, P. Plummer, Q. Zhang, and S. Ryu Role of the DksA-Like Protein in the Pathogenesis and Diverse Metabolic Activity of Campylobacter jejuni J. Bacteriol., July 1, 2008; 190(13): 4512 - 4520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kutter, R. Buhrdorf, J. Haas, W. Schneider-Brachert, R. Haas, and W. Fischer Protein Subassemblies of the Helicobacter pylori Cag Type IV Secretion System Revealed by Localization and Interaction Studies J. Bacteriol., March 15, 2008; 190(6): 2161 - 2171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. A. M. van Hoek, S. Mayrhofer, K. J. Domig, A. B. Florez, M. S. Ammor, B. Mayo, and H. J. M. Aarts Mosaic Tetracycline Resistance Genes and Their Flanking Regions in Bifidobacterium thermophilum and Lactobacillus johnsonii Antimicrob. Agents Chemother., January 1, 2008; 52(1): 248 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Poly, T. Read, D. R. Tribble, S. Baqar, M. Lorenzo, and P. Guerry Genome Sequence of a Clinical Isolate of Campylobacter jejuni from Thailand Infect. Immun., July 1, 2007; 75(7): 3425 - 3433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wardak, J. Szych, A. A. Zasada, and R. Gierczynski Antibiotic Resistance of Campylobacter jejuni and Campylobacter coli Clinical Isolates from Poland Antimicrob. Agents Chemother., March 1, 2007; 51(3): 1123 - 1125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. A. Oyarzabal, R. Rad, and S. Backert Conjugative Transfer of Chromosomally Encoded Antibiotic Resistance from Helicobacter pylori to Campylobacter jejuni J. Clin. Microbiol., February 1, 2007; 45(2): 402 - 408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Parker, B. Quinones, W. G. Miller, S. T. Horn, and R. E. Mandrell Comparative Genomic Analysis of Campylobacter jejuni Strains Reveals Diversity Due to Genomic Elements Similar to Those Present in C. jejuni Strain RM1221 J. Clin. Microbiol., November 1, 2006; 44(11): 4125 - 4135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hofreuter, J. Tsai, R. O. Watson, V. Novik, B. Altman, M. Benitez, C. Clark, C. Perbost, T. Jarvie, L. Du, et al. Unique Features of a Highly Pathogenic Campylobacter jejuni Strain. Infect. Immun., August 1, 2006; 74(8): 4694 - 4707. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Grant, C. Coward, M. A. Jones, C. A. Woodall, P. A. Barrow, and D. J. Maskell Signature-Tagged Transposon Mutagenesis Studies Demonstrate the Dynamic Nature of Cecal Colonization of 2-Week-Old Chickens by Campylobacter jejuni Appl. Envir. Microbiol., December 1, 2005; 71(12): 8031 - 8041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Nirdnoy, C. J. Mason, and P. Guerry Mosaic Structure of a Multiple-Drug-Resistant, Conjugative Plasmid from Campylobacter jejuni Antimicrob. Agents Chemother., June 1, 2005; 49(6): 2454 - 2459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Poly, D. Threadgill, and A. Stintzi Genomic Diversity in Campylobacter jejuni: Identification of C. jejuni 81-176-Specific Genes J. Clin. Microbiol., May 1, 2005; 43(5): 2330 - 2338. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
