| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 E0364 Inserm – Université Lille II (Faculté de Médecine Henri Warembourg) – Institut Pasteur de Lille, Lille, France
2 U629 Inserm – Institut Pasteur de Lille, Lille, France
3 Division of Biophysics, Research Center Borstel, Borstel, Germany
4 Unidad de Investigacion, Hospital Son Dureta and Institut Universitari d'Investigació en Ciències de la Salut (IUNICS), Palma Mallorca, Spain
Correspondence
M. Marceau
michael.marceau{at}ibl.fr
The Yersinia pseudotuberculosis chromosome contains a seven-gene polycistronic unit (the pmrF operon) whose products share extensive homologies with their pmrF counterparts in Salmonella enterica serovar Typhimurium (S. typhimurium), another Gram-negative bacterial enteropathogen. This gene cluster is essential for addition of 4-aminoarabinose to the lipid moiety of LPS, as demonstrated by MALDI-TOF mass spectrometry of lipid A from both wild-type and pmrF-mutated strains. As in S. typhimurium, 4-aminoarabinose substitution of lipid A contributes to in vitro resistance of Y. pseudotuberculosis to the antimicrobial peptide polymyxin B. Whereas pmrF expression in S. typhimurium is mediated by both the PhoP–PhoQ and PmrA–PmrB two-component regulatory systems, it appears to be PmrA–PmrB-independent in Y. pseudotuberculosis, with the response regulator PhoP interacting directly with the pmrF operon promoter region. This result reveals that the ubiquitous PmrA–PmrB regulatory system controls different regulons in distinct bacterial species. In addition, pmrF inactivation in Y. pseudotuberculosis has no effect on bacterial virulence in the mouse, again in contrast to the situation in S. typhimurium. The marked differences in pmrF operon regulation in these two phylogenetically close bacterial species may be related to their dissimilar lifestyles.
These authors contributed equally to this work.
The GenBank/EMBL/DDBJ accession numbers for the Y. pseudotuberculosis pmrF, phoP–phoQ and pmrA–pmrB operons are AF336802, AF333125 and AY259243 respectively.
This article has been cited by other articles:
|
|
 |
|
  W.-B. Wang, I-C. Chen, S.-S. Jiang, H.-R. Chen, C.-Y. Hsu, P.-R. Hsueh, W.-B. Hsu, and S.-J. Liaw Role of RppA in the Regulation of Polymyxin B Susceptibility, Swarming, and Virulence Factor Expression in Proteus mirabilis Infect. Immun., May 1, 2008; 76(5): 2051 - 2062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-W. Lee, K.-S. Jeong, S.-W. Han, S.-E. Lee, B.-K. Phee, T.-R. Hahn, and P. Ronald The Xanthomonas oryzae pv. oryzae PhoPQ Two-Component System Is Required for AvrXA21 Activity, hrpG Expression, and Virulence J. Bacteriol., March 15, 2008; 190(6): 2183 - 2197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Fisher, C. Castillo, and J. Mecsas Intranasal Inoculation of Mice with Yersinia pseudotuberculosis Causes a Lethal Lung Infection That Is Dependent on Yersinia Outer Proteins and PhoP Infect. Immun., January 1, 2007; 75(1): 429 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Grabenstein, H. S. Fukuto, L. E. Palmer, and J. B. Bliska Characterization of Phagosome Trafficking and Identification of PhoP-Regulated Genes Important for Survival of Yersinia pestis in Macrophages Infect. Immun., July 1, 2006; 74(7): 3727 - 3741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Erickson, C. O. Jarrett, B. W. Wren, and B. J. Hinnebusch Serotype Differences and Lack of Biofilm Formation Characterize Yersinia pseudotuberculosis Infection of the Xenopsylla cheopis Flea Vector of Yersinia pestis J. Bacteriol., February 1, 2006; 188(3): 1113 - 1119. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
