HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lindbäck, T. Articles by Granum, P. E. Search for Related Content PubMed PubMed Citation Articles by Lindbäck, T. Articles by Granum, P. E. Agricola Articles by Lindbäck, T. Articles by Granum, P. E.

Characterization of the Bacillus cereus Nhe enterotoxin

Marianne Skeie Rødland

Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, PO Box 8146 Dep., N-0033 Oslo, Norway

Correspondence
Per Einar Granum
per.e.granum{at}veths.no

The non-haemolytic enterotoxin (Nhe) is one of two three-component enterotoxins responsible for the diarrhoeal food-poisoning syndrome caused by Bacillus cereus. Nhe is composed of NheA, NheB and NheC. The three genes encoding the Nhe components constitute an operon, and the transcriptional start site is located 61 bp upstream of the nheA translational start site. The nhe genes were cloned separately, and expressed in either Bacillus subtilis or Escherichia coli. Separate expression showed that all three components were required for biological activity. In addition, NheA and NheB were purified from B. cereus culture supernatants. As NheC seems to be expressed in only small amounts by B. cereus, NheC was expressed and purified as a histidine-tagged fusion protein. The maximum cytotoxic activity was obtained when the molar ratio between NheA : NheB : His₆-NheC was 10 : 10 : 1, and it was shown that NheB was the binding component of the enterotoxin complex.

Present address: Faculty of Medicine, Rikshospitalet, University of Oslo, N-0027 Oslo, Norway.

This article has been cited by other articles:

				A. Fagerlund, T. Lindback, A. K. Storset, P. E. Granum, and S. P. Hardy Bacillus cereus Nhe is a pore-forming toxin with structural and functional properties similar to the ClyA (HlyE, SheA) family of haemolysins, able to induce osmotic lysis in epithelia Microbiology, March 1, 2008; 154(3): 693 - 704. [Abstract] [Full Text] [PDF]

				C. Duport, A. Zigha, E. Rosenfeld, and P. Schmitt Control of Enterotoxin Gene Expression in Bacillus cereus F4430/73 Involves the Redox-Sensitive ResDE Signal Transduction System. J. Bacteriol., September 1, 2006; 188(18): 6640 - 6651. [Abstract] [Full Text] [PDF]

				K. Frederiksen, H. Rosenquist, K. Jorgensen, and A. Wilcks Occurrence of Natural Bacillus thuringiensis Contaminants and Residues of Bacillus thuringiensis-Based Insecticides on Fresh Fruits and Vegetables. Appl. Envir. Microbiol., May 1, 2006; 72(5): 3435 - 3440. [Abstract] [Full Text] [PDF]

				R. Dietrich, M. Moravek, C. Burk, P. E. Granum, and E. Martlbauer Production and Characterization of Antibodies against Each of the Three Subunits of the Bacillus cereus Nonhemolytic Enterotoxin Complex Appl. Envir. Microbiol., December 1, 2005; 71(12): 8214 - 8220. [Abstract] [Full Text] [PDF]