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Microbiology 150 (2004), 4075-4084; DOI  10.1099/mic.0.27472-0
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Microbiology 150 (2004), 4075-4084; DOI  10.1099/mic.0.27472-0
© 2004 Society for General Microbiology

Physical and genetic map of the Weissella paramesenteroides DSMZ 20288T chromosome and characterization of different rrn operons by ITS analysis

Ivo M. Chelo, Líbia Zé-Zé, Lélia Chambel and Rogério Tenreiro

Universidade de Lisboa, Faculdade de Ciências, Centro de Genética e Biologia Molecular and Instituto de Ciência Aplicada e Tecnologia, Edifício ICAT, Campus da FCUL, Campo Grande, 1749-016 Lisboa, Portugal

Correspondence
Rogério Tenreiro
rptenreiro{at}fc.ul.pt

The Weissella paramesenteroides DSMZ 20288T chromosome was analysed by pulsed-field gel electrophoresis, enabling the construction of a physical and genetic map. A total of 21 recognition sites of the restriction enzymes AscI, I-CeuI, NotI and SfiI were mapped on the chromosome, which was found to be circular with an estimated size of 2026 kb. This is believed to constitute the first study into the genomic organization of a strain of this genus, addressing the localization of important chromosomal regions such as oriC and terC. A total of 23 genetic markers were mapped, including eight rrn operons that were precisely assigned in 37 % of the W. paramesenteroides chromosome. The transcription direction of rrn loci was determined and three different rrn clusters were recognized regarding the presence/absence of tRNA genes in ITS regions.

Abbreviations: CHEF, counter-clamped homogeneous electric field; ITS, internal transcribed spacer; LAB, lactic acid bacteria

The GenBank accession numbers for the sequences reported in this paper are given in Tables 1 and 2 and in the legend of Fig. 3.






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