Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 150 (2004), 4147-4156; DOI  10.1099/mic.0.27498-0
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ughy, B.
Right arrow Articles by Ajlani, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ughy, B.
Right arrow Articles by Ajlani, G.
Agricola
Right arrow Articles by Ughy, B.
Right arrow Articles by Ajlani, G.
Microbiology 150 (2004), 4147-4156; DOI  10.1099/mic.0.27498-0
© 2004 Society for General Microbiology

Phycobilisome rod mutants in Synechocystis sp. strain PCC6803

Bettina Ughy{dagger} and Ghada Ajlani

Département de Biologie Joliot-Curie, SBFM, CEA Saclay, F-91191, Gif-sur-Yvette, France

Correspondence
Ghada Ajlani
gajlani{at}cea.fr

The phycobilisome is a large pigment-protein assembly that harvests light energy for photosynthesis. This supramolecular complex is composed of two main structures: a core substructure and peripheral rods. Linker polypeptides assemble phycobiliproteins within these structures and optimize light absorption and energy transfer. Mutations have been constructed in three rod-linker-coding genes located in the cpc operon of Synechocystis sp. strain PCC6803. The cpcC1 gene encoding the 33 kDa linker is found to be epistatic to cpcC2 encoding the 30 kDa linker, indicating a specific role for each of these two linkers in rod growth. This corroborates studies on the sequential degradation of phycobilisomes upon nitrogen starvation. Three allelic mutants affecting cpcC2 revealed a polar effect of commonly used cassettes (aphI, aadA) on the operon steady-state transcripts and an effect of rod linker availability on the amount of phycocyanin incorporated in the phycobilisome. This led to the proposal that regulation of rod length could occur through processing of transcripts upstream of the cpcC2 gene.

Abbreviations: AP, allophycocyanin; FNR, ferredoxin NADP+ reductase; Lx, linker polypeptide located at position x of the phycobilisome, where x can be R (rod), RC (rod core) or CM (core membrane), linkers with identical location are distinguished by a superscript just above the x indicating their molecular mass; PC, phycocyanin; PBS, phycobilisome

{dagger}Present address: Institute of Plant Biology, BRC, Temesvári Krt. 62, H-6701, Szeged, Hungary.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J.-C. Thomas, B. Ughy, B. Lagoutte, and G. Ajlani
A second isoform of the ferredoxin:NADP oxidoreductase generated by an in-frame initiation of translation
PNAS, November 28, 2006; 103(48): 18368 - 18373.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2004 Society for General Microbiology.