HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Pardo, M. Articles by Gil, C. Search for Related Content PubMed PubMed Citation Articles by Pardo, M. Articles by Gil, C. Agricola Articles by Pardo, M. Articles by Gil, C.

PST1 and ECM33 encode two yeast cell surface GPI proteins important for cell wall integrity

Mercedes Pardo

Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense, Pza. Ramón y Cajal s/n, 28040 Madrid, Spain

Correspondence
Concha Gil
conchagil{at}farm.ucm.es

Pst1p was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction. ECM33 encodes a protein homologous to Pst1p, and both of them display typical features of GPI-anchored proteins and a characteristic receptor L-domain. Pst1p and Ecm33p are both localized to the cell surface, Pst1p being at the cell membrane and possibly also in the periplasmic space. Here, the characterization of pst1, ecm33 and pst1 ecm33 mutants is described. Deletion of ECM33 leads to a weakened cell wall, and this defect is further aggravated by simultaneous deletion of PST1. As a result, the ecm33 mutant displays increased levels of activated Slt2p, the MAP kinase of the cell integrity pathway, and relies on a functional Slt2-mediated cell integrity pathway to ensure viability. Analyses of model glycosylated proteins show glycosylation defects in the ecm33 mutant. Ecm33p is also important for proper cell wall ultrastructure organization and, furthermore, for the correct assembly of the mannoprotein outer layer of the cell wall. Pst1p seems to act in the compensatory mechanism activated upon cell wall damage and, in these conditions, may partially substitute for Ecm33p.

This paper is dedicated to the memory of the Spanish scientist Dr David Vázquez (1931–1986).

Present address: Proteomic Mass Spectrometry, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

This article has been cited by other articles:

				R. J. Luallen, J. Lin, H. Fu, K. K. Cai, C. Agrawal, I. Mboudjeka, F.-H. Lee, D. Montefiori, D. F. Smith, R. W. Doms, et al. An Engineered Saccharomyces cerevisiae Strain Binds the Broadly Neutralizing Human Immunodeficiency Virus Type 1 Antibody 2G12 and Elicits Mannose-Specific gp120-Binding Antibodies J. Virol., July 1, 2008; 82(13): 6447 - 6457. [Abstract] [Full Text] [PDF]

				L. E. Cowen and W. J. Steinbach Stress, Drugs, and Evolution: the Role of Cellular Signaling in Fungal Drug Resistance Eukaryot. Cell, May 1, 2008; 7(5): 747 - 764. [Full Text] [PDF]

				G. Pardini, P. W. J. De Groot, A. T. Coste, M. Karababa, F. M. Klis, C. G. de Koster, and D. Sanglard The CRH Family Coding for Cell Wall Glycosylphosphatidylinositol Proteins with a Predicted Transglycosidase Domain Affects Cell Wall Organization and Virulence of Candida albicans J. Biol. Chem., December 29, 2006; 281(52): 40399 - 40411. [Abstract] [Full Text] [PDF]

				J. Romano, G. Nimrod, N. Ben-Tal, Y. Shadkchan, K. Baruch, H. Sharon, and N. Osherov Disruption of the Aspergillus fumigatus ECM33 homologue results in rapid conidial germination, antifungal resistance and hypervirulence Microbiology, July 1, 2006; 152(7): 1919 - 1928. [Abstract] [Full Text] [PDF]

				S. Chabane, J. Sarfati, O. Ibrahim-Granet, C. Du, C. Schmidt, I. Mouyna, M.-C. Prevost, R. Calderone, and J.-P. Latge Glycosylphosphatidylinositol-Anchored Ecm33p Influences Conidial Cell Wall Biosynthesis in Aspergillus fumigatus. Appl. Envir. Microbiol., May 1, 2006; 72(5): 3259 - 3267. [Abstract] [Full Text] [PDF]

				S. M. Bowman, A. Piwowar, M. Al Dabbous, J. Vierula, and S. J. Free Mutational Analysis of the Glycosylphosphatidylinositol (GPI) Anchor Pathway Demonstrates that GPI-Anchored Proteins Are Required for Cell Wall Biogenesis and Normal Hyphal Growth in Neurospora crassa Eukaryot. Cell, March 1, 2006; 5(3): 587 - 600. [Abstract] [Full Text] [PDF]

				A. Albrecht, A. Felk, I. Pichova, J. R. Naglik, M. Schaller, P. de Groot, D. MacCallum, F. C. Odds, W. Schafer, F. Klis, et al. Glycosylphosphatidylinositol-anchored Proteases of Candida albicans Target Proteins Necessary for Both Cellular Processes and Host-Pathogen Interactions J. Biol. Chem., January 13, 2006; 281(2): 688 - 694. [Abstract] [Full Text] [PDF]

				R. Martinez-Lopez, H. Park, C. L. Myers, C. Gil, and S. G. Filler Candida albicans Ecm33p Is Important for Normal Cell Wall Architecture and Interactions with Host Cells Eukaryot. Cell, January 1, 2006; 5(1): 140 - 147. [Abstract] [Full Text] [PDF]

				A. M. Neiman Ascospore Formation in the Yeast Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., December 1, 2005; 69(4): 565 - 584. [Abstract] [Full Text] [PDF]

				Q. Y. Yin, P. W. J. de Groot, H. L. Dekker, L. de Jong, F. M. Klis, and C. G. de Koster Comprehensive Proteomic Analysis of Saccharomyces cerevisiae Cell Walls: IDENTIFICATION OF PROTEINS COVALENTLY ATTACHED VIA GLYCOSYLPHOSPHATIDYLINOSITOL REMNANTS OR MILD ALKALI-SENSITIVE LINKAGES J. Biol. Chem., May 27, 2005; 280(21): 20894 - 20901. [Abstract] [Full Text] [PDF]