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Microbiology 150 (2004), 365-371; DOI  10.1099/mic.0.26826-0
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Microbiology 150 (2004), 365-371; DOI  10.1099/mic.0.26826-0
© 2004 Society for General Microbiology

Mouse skin passage of Streptococcus pyogenes results in increased streptokinase expression and activity

Myrna S. Rezcallah1, Michael D. P. Boyle2 and Darren D. Sledjeski3

1 Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
2 Department of Biology, Juniata College, 1700 Moore Street, Huntingdon, PA 16652, USA
3 Department of Microbiology and Immunology, Medical College of Ohio, 3055 Arlington Avenue, Toledo, OH 43614-5806, USA

Correspondence
Darren D. Sledjeski
dsledjeski{at}mco.edu

The plasminogen activator streptokinase has been proposed to be a key component of a complex mechanism that promotes skin invasion by Streptococcus pyogenes. This study was designed to compare ska gene message and protein levels in wild-type M1 serotype isolate 1881 and a more invasive variant recovered from the spleen of a lethally infected mouse. M1 isolates selected for invasiveness demonstrated enhanced levels of active plasminogen activator activity in culture. This effect was due to a combination of increased expression of the ska gene and decreased expression of the speB gene. The speB gene product, SpeB, was found to efficiently degrade streptokinase in vitro.

Abbreviations: QRT-PCR, quantitative real-time reverse transcriptase-PCR; SELDI-TOF, surface-enhanced laser desorption ionization–time of flight; SK, streptokinase




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