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Pseudomonas syringae pv. phaseolicola can be separated into two genetic lineages distinguished by the possession of the phaseolotoxin biosynthetic cluster

Laurent Sutra^2,

¹ Instituto de Agrobiotecnología y Recursos Naturales, CSIC-UPNA, and Laboratorio de Patología Vegetal, Departamento de Producción Agraria, Universidad Pública de Navarra, 31006 Pamplona, Spain
² UMR de Pathologie Végétale INRA-INH-Université, Beaucouzé, 49071 France
³ Centre for Research in Plant Science, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK

Correspondence
Jesús Murillo
jesus{at}unavarra.es

The bean (Phaseolus spp.) plant pathogen Pseudomonas syringae pv. phaseolicola is characterized by the ability to produce phaseolotoxin (Tox⁺). We recently reported that the majority of the Spanish P. syringae pv. phaseolicola population is unable to synthesize this toxin (Tox^-). These Tox^- isolates appear to lack the entire DNA region for the biosynthesis of phaseolotoxin (argK-tox gene cluster), as shown by PCR amplification and DNA hybridization using DNA sequences specific for separated genes of this cluster. Tox⁺ and Tox^- isolates also showed genomic divergence that included differences in ERIC-PCR and arbitrarily primed-PCR profiles. Tox⁺ isolates showed distinct patterns of IS801 genomic insertions and contained a chromosomal IS801 insertion that was absent from Tox^- isolates. Using a heteroduplex mobility assay, sequence differences were observed only among the intergenic transcribed spacer of the five rDNA operons of the Tox^- isolates. The techniques used allowed the unequivocal differentiation of isolates of P. syringae pv. phaseolicola from the closely related soybean (Glycine max) pathogen, P. syringae pv. glycinea. Finally, a pathogenicity island that is essential for the pathogenicity of P. syringae pv. phaseolicola on beans appears to be conserved among Tox⁺, but not among Tox^- isolates, which also lacked the characteristic large plasmid that carries this pathogenicity island. It is proposed that the results presented here justify the separation of the Tox⁺ and Tox^- P. syringae pv. phaseolicola isolates into two distinct genetic lineages, designated Pph1 and Pph2, respectively, that show relevant genomic differences that include the pathogenicity gene complement.

Deceased (d. 16 December 2002); this paper is dedicated to his memory.

The EMBL accession numbers for the sequences reported in this paper are AJ568000 (IS50, 734 bp), AJ568001 (IS50, 295 bp), AJ568002 (ERIC, 1289 bp), AJ550186 (EEL-Pph1), AJ550187 (EEL-Pph2) and AJ550188 (EEL-Pseudomonas syringae pv. glycinea).