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Microbiology 150 (2004), 707-713; DOI  10.1099/mic.0.26707-0
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Microbiology 150 (2004), 707-713; DOI  10.1099/mic.0.26707-0
© 2004 Society for General Microbiology

Formaldehyde dehydrogenase preparations from Methylococcus capsulatus (Bath) comprise methanol dehydrogenase and methylene tetrahydromethanopterin dehydrogenase

Ekundayo K. Adeosun1,{dagger}, Thomas J. Smith1,{ddagger}, Anne-Mette Hoberg1,§, Giles Velarde2, Robert Ford2 and Howard Dalton1

1 Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
2 Department of Biomolecular Sciences, PO Box 88, UMIST, Manchester M60 1QD, UK

Correspondence
Howard Dalton
H.Dalton{at}warwick.ac.uk

In methylotrophic bacteria, formaldehyde is an important but potentially toxic metabolic intermediate that can be assimilated into biomass or oxidized to yield energy. Previously reported was the purification of an NAD(P)+-dependent formaldehyde dehydrogenase (FDH) from the obligate methane-oxidizing methylotroph Methylococcus capsulatus (Bath), presumably important in formaldehyde oxidation, which required a heat-stable factor (known as the modifin) for FDH activity. Here, the major protein component of this FDH preparation was shown by biophysical techniques to comprise subunits of 64 and 8 kDa in an {alpha}2{beta}2 arrangement. N-terminal sequencing of the subunits of FDH, together with enzymological characterization, showed that the {alpha}2{beta}2 tetramer was a quinoprotein methanol dehydrogenase of the type found in other methylotrophs. The FDH preparations were shown to contain a highly active NAD(P)+-dependent methylene tetrahydromethanopterin dehydrogenase that was the probable source of the NAD(P)+-dependent formaldehyde oxidation activity. These results support previous findings that methylotrophs possess multiple pathways for formaldehyde dissimilation.

Abbreviations: ES-MS, electrospray-mass spectrometry; FDH, formaldehyde dehydrogenase; H4MPT, tetrahydromethanopterin; HTSE, heat-treated soluble extract; MDH, methanol dehydrogenase; methylene H4MPT-DH, methylene tetrahydromethanopterin dehydrogenase

{dagger}Present address: GlaxoSmithkline Plc, New Frontiers Science Park, Harlow, Essex CM19 5AW, UK.

{ddagger}Present address: Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, UK.

§Present address: Dept Clinical Pharmacology Q7642, Rigshospitalet, Blegdamsvej 9, DK-2100 Copenhagen Ø, Denmark.






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