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Microbiology 150 (2004), 1023-1029; DOI  10.1099/mic.0.26629-0
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Microbiology 150 (2004), 1023-1029; DOI  10.1099/mic.0.26629-0
© 2004 Society for General Microbiology

A putative transposase gene in the 16S–23S rRNA intergenic spacer region of Mycoplasma imitans

Ryô Harasawa1, David G. Pitcher2, Ana S. Ramírez3,{dagger} and Janet M. Bradbury3

1 Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan
2 Respiratory and Systemic Infection Laboratory, Health Protection Agency, 61 Colindale Avenue, London NW9 5HT, UK
3 Department of Veterinary Pathology, The University of Liverpool, Jordan Bldg, Leahurst, Neston CH64 7TE, UK

Correspondence
Ryô Harasawa
ryo{at}m.u-tokyo.ac.jp

Examination of the nucleotide sequences of the 16S–23S intergenic transcribed spacer (ITS) region of Mycoplasma imitans and Mycoplasma gallisepticum identified a putative transposase gene located only in the ITS of M. imitans, which can be used as a genetic marker to distinguish these two species. The relative size of the PCR products of the ITS region allowed a clear distinction to be made between strains of M. imitans and M. gallisepticum, both of which could be readily discriminated from the type strains of all the other recognized avian Mycoplasma species. In addition, the putative transposase gene assigned in the ITS of M. imitans was shown to include a sequence homologous to that of the P75 gene of M. gallisepticum. This is believed to be the first description of an insertion element in the rRNA operon region of a mycoplasma species.

Abbreviations: ITS, intergenic transcribed spacer

The GenBank accession numbers for the new sequences first reported in this paper are AB098503 and AB098504.

{dagger}Present address: Sección de Epidemiología y Medicina Preventiva, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, Trasmontaña s/n 35416 Arucas, Spain.




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