| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Biology, The University, D-78457 Konstanz, Germany
Correspondence
Alasdair Cook
alasdair.cook{at}uni-konstanz.de
Growth of the 
-proteobacterium Paracoccus denitrificans NKNIS with taurine or isethionate as sole source of carbon involves sulfoacetaldehyde acetyltransferase (Xsc), which is presumably encoded by an xsc gene in subgroup 3, none of whose gene products has been characterized. The genome of the -proteobacterium Rhodobacter sphaeroides 2.4.1 was interpreted to contain a nine-gene cluster encoding the inducible dissimilation of taurine, and this deduced pathway included a regulator, a tripartite ATP-independent transporter, taurine dehydrogenase (TDH; presumably TauXY) as well as Xsc (subgroup 3), a hypothetical protein and phosphate acetyltransferase (Pta). A similar cluster was found in P. denitrificans NKNIS, in contrast to an analogous cluster encoding an ATP-binding cassette transporter in Paracoccus pantotrophus. Inducible TDH, Xsc and Pta were found in extracts of taurine-grown cells of strain NKNIS. TDH oxidized taurine to sulfoacetaldehyde and ammonium ion with cytochrome c as electron acceptor. Whereas Xsc and Pta were soluble enzymes, TDH was located in the particulate fraction, where inducible proteins with the expected masses of TauXY (14 and 50 kDa, respectively) were detected by SDS-PAGE. Xsc and Pta were separated by anion-exchange chromatography. Xsc was effectively pure; the molecular mass of the subunit (64 kDa) and the N-terminal amino acid sequence confirmed the identification of the xsc gene. Inducible isethionate dehydrogenase (IDH), Xsc and Pta were assayed in extracts of isethionate-grown cells of strain NKNIS. IDH was located in the particulate fraction, oxidized isethionate to sulfoacetaldehyde with cytochrome c as electron acceptor and correlated with the expression of a 62 kDa protein. Strain NKNIS excreted sulfite and sulfate during growth with a sulfonate and no sulfite dehydrogenase was detected. There is considerable biochemical, genetic and regulatory complexity in the degradation of these simple molecules.
The GenBank accession numbers for the sequences reported in this paper are AY498613 (gene cluster in Paracoccus denitrificans NKNIS), AY498615 (gene cluster in Paracoccus pantotrophus NKNCYSA), AY498614 (gene cluster in P. pantotrophus DSM 65), AY498616 (partial tauY in Achromobacter xylosoxidans NCIMB 10751), AY498617 (partial tauY in Comamonas sp. strain SFCD1) and AY498618 (partial tauY in Ralstonia sp. strain EDS1).
Present address: Institut für Biologie der Freien Universität Berlin, D-14195 Berlin, Germany.
This article has been cited by other articles:
|
|
 |
|
  J. Wiethaus, B. Schubert, Y. Pfander, F. Narberhaus, and B. Masepohl The GntR-Like Regulator TauR Activates Expression of Taurine Utilization Genes in Rhodobacter capsulatus J. Bacteriol., January 15, 2008; 190(2): 487 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Weinitschke, K. Denger, A. M. Cook, and T. H. M. Smits The DUF81 protein TauE in Cupriavidus necator H16, a sulfite exporter in the metabolism of C2 sulfonates Microbiology, September 1, 2007; 153(9): 3055 - 3060. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Denger, T. H. M. Smits, and A. M. Cook Genome-enabled analysis of the utilization of taurine as sole source of carbon or of nitrogen by Rhodobacter sphaeroides 2.4.1. Microbiology, November 1, 2006; 152(Pt 11): 3197 - 3206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Stiens, S. Schneiker, M. Keller, S. Kuhn, A. Puhler, and A. Schluter Sequence Analysis of the 144-Kilobase Accessory Plasmid pSmeSM11a, Isolated from a Dominant Sinorhizobium meliloti Strain Identified during a Long-Term Field Release Experiment. Appl. Envir. Microbiol., May 1, 2006; 72(5): 3662 - 3672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Weinitschke, K. Denger, T. H. M. Smits, K. Hollemeyer, and A. M. Cook The sulfonated osmolyte N-methyltaurine is dissimilated by Alcaligenes faecalis and by Paracoccus versutus with release of methylamine. Microbiology, April 1, 2006; 152(Pt 4): 1179 - 1186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. H. Thomas, T. Southworth, M. R. Leon-Kempis, A. Leech, and D. J. Kelly Novel ligands for the extracellular solute receptors of two bacterial TRAP transporters Microbiology, January 1, 2006; 152(1): 187 - 198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. N. Erwin, S. Nakano, and P. Zuber Sulfate-Dependent Repression of Genes That Function in Organosulfur Metabolism in Bacillus subtilis Requires Spx J. Bacteriol., June 15, 2005; 187(12): 4042 - 4049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Weinitschke, K. S. von Rekowski, K. Denger, and A. M. Cook Sulfoacetaldehyde is excreted quantitatively by Acinetobacter calcoaceticus SW1 during growth with taurine as sole source of nitrogen Microbiology, April 1, 2005; 151(4): 1285 - 1290. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Wolfe The Acetate Switch Microbiol. Mol. Biol. Rev., March 1, 2005; 69(1): 12 - 50. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Rein, R. Gueta, K. Denger, J. Ruff, K. Hollemeyer, and A. M. Cook Dissimilation of cysteate via 3-sulfolactate sulfo-lyase and a sulfate exporter in Paracoccus pantotrophus NKNCYSA Microbiology, March 1, 2005; 151(3): 737 - 747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Denger, J. Ruff, D. Schleheck, and A. M. Cook Rhodococcus opacus expresses the xsc gene to utilize taurine as a carbon source or as a nitrogen source but not as a sulfur source Microbiology, June 1, 2004; 150(6): 1859 - 1867. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
