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Translocation of transposition-deficient (Tn^dPKLH2-like) transposons in the natural environment: mechanistic insights from the study of adjacent DNA sequences

¹ Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia
² School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK

Correspondence
Gennady Kholodii
kholodii{at}img.ras.ru

A family of plasmid-borne DNA fragments of different length, apparently inherited from an ancient plasmid, has been identified in the world population of environmental Acinetobacter strains. These fragments, named PPFs (parental plasmid DNA fragments), were 99·8 % identical to each other in the common regions, and contained in their central region a variant of an aberrant mercury-resistance transposon (Tn^dPKLH2) that has lost its transposition genes. As a rule, recombinogenic elements were found at the breakpoints of identity between the different PPFs. Of these recombinogenic elements, a newly identified IS6 family element, a transposon, or a resolvase gene interrupted one end of the PPFs. At the opposite end, the breakpoint of some PPFs was mapped to the recombination point within, in each case, a different variant of a res site (RS2), whilst in other PPFs, this end was eroded by insertion of a newly identified IS6 family element. On the basis of DNA sequence data, possible mechanisms of translocation of defective Tn^dPKLH2-like elements via recombination events implicating the nearby res (resolution) site and IS element are proposed.

This work is dedicated to the memory of Roman B. Khesin.

The accession numbers for the nucleotide sequences reported in this work are: AF213017 (pKLH2); AJ487050 and AJ251126 (pKLH204); AJ251306 and AJ251307 (pKLH201); AJ486855 (pKLH203); AJ486857 (pKLH202); AJ250171 and AJ486856 (pKLH207); AJ459234 (pKLH205); AJ251272 (pKLH208).

A table showing genetic similarities for the Acinetobacter determinants identified within the regions flanking the mercury-resistant loci is available as supplementary data with the online version of this paper (at http://mic.sgmjournals.org).

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