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Microbiology 150 (2004), 1245-1250; DOI  10.1099/mic.0.26923-0
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Microbiology 150 (2004), 1245-1250; DOI  10.1099/mic.0.26923-0
© 2004 Society for General Microbiology

Mitosomes of Entamoeba histolytica are abundant mitochondrion-related remnant organelles that lack a detectable organellar genome

Gloria León-Avila{dagger} and Jorge Tovar

School of Biological Sciences, Royal Holloway, University of London, Egham TW20 0EX, UK

Correspondence
Jorge Tovar
j.tovar{at}rhul.ac.uk

The existence of mitochondrion-related relict organelles (mitosomes) in the amitochondrial human pathogen Entamoeba histolytica and the detection of extranuclear DNA-containing cytoplasmic structures (EhKOs) has led to the suggestion that a remnant genome from the original mitochondrial endosymbiont might have been retained in this organism. This study reports on the mutually exclusive distribution of Cpn60 and extranuclear DNA in E. histolytica and on the distribution of Cpn60-containing mitosomes in this parasite. In situ nick-translation coupled to immunofluorescence microscopy failed to detect the presence of DNA in mitosomes, either in fixed parasite trophozoites or in partially purified organellar fractions. These results indicate that a remnant organellar genome has not been retained in E. histolytica mitosomes and demonstrate unequivocally that EhKOs and mitosomes are distinct and unrelated cellular structures.

Abbreviations: EhKO, Entamoeba histolytica kinetoplast-like organelle

{dagger}Present address: Departmento de Genética y Biología Molecular, Centro de Investigación y Estudios Avanzados I.P.N., San Pedro Zacatenco, 07360, Mexico.




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