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Global analysis of gene expression in an rpoN mutant of Listeria monocytogenes

¹ Equipe de Microbiologie Fondamentale et Appliquée, Université de Poitiers, 40 avenue du Recteur Pineau, 86022 Poitiers Cedex, France
² Laboratoire de Génomique des Micro-organismes Pathogènes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
³ Station de Recherches sur la Viande, Institut National de la Recherche Agronomique de Theix, 63122 Saint-Genes Champanelle, France
⁴ Plateforme de protéomique, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France

Correspondence
Yann Héchard
yann.hechard{at}univ-poitiers.fr

The role of the alternative ⁵⁴ factor, encoded by the rpoN gene, was investigated in Listeria monocytogenes by comparing the global gene expression of the wild-type EGDe strain and an rpoN mutant. Gene expression, using whole-genome macroarrays, and protein content, using two-dimensional gel electrophoresis, were analysed. Seventy-seven genes and nine proteins, whose expression was modulated in the rpoN mutant as compared to the wild-type strain, were identified. Most of the modifications were related to carbohydrate metabolism and in particular to pyruvate metabolism. However, under the conditions studied, only the mptACD operon was shown to be directly controlled by ⁵⁴. Therefore, the remaining modifications seem to be due to indirect effects. In parallel, an in silico analysis suggests that ⁵⁴ may directly control the expression of four different phosphotransferase system (PTS) operons, including mptACD. PTS activity is known to have a direct effect on the pyruvate pool and on catabolite regulation. These results suggest that ⁵⁴ is mainly involved in the control of carbohydrate metabolism in L. monocytogenes via direct regulation of PTS activity, alteration of the pyruvate pool and modulation of carbon catabolite regulation.

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