HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gomez-Gil, B. Articles by Swings, J. Search for Related Content PubMed PubMed Citation Articles by Gomez-Gil, B. Articles by Swings, J. Agricola Articles by Gomez-Gil, B. Articles by Swings, J.

Molecular identification of Vibrio harveyi-related isolates associated with diseased aquatic organisms

¹ CIAD/Mazatlán Unit for Aquaculture, AP 711, Mazatlán Sinaloa, Mexico 82000
² CIBNOR, AP 128, La Paz, Baja California, Mexico 23090
³ Laboratory for Microbiology, K. L. Ledeganckstraat 35, Ghent University, Ghent 9000, Belgium

Correspondence
Bruno Gomez-Gil
bruno{at}victoria.ciad.mx

Fifty strains belonging to Vibrio harveyi, Vibrio campbellii, and the recently described Vibrio rotiferianus, were analysed using phenotypic and genomic techniques with the aim of analysing the usefulness of the different techniques for the identification of V. harveyi-related species. The species V. harveyi and V. campbellii were phenotypically indistinguishable by more than 100 phenotypic features. Thirty-nine experimental strains were phenotypically identified as V. harveyi, but FAFLP, REP-PCR, IGS-PCR and DNA–DNA hybridization proved that they in fact belong to the species V. campbellii. Similar groupings were found among all fingerprinting methodologies (except IGS-PCR). Thirty-two experimental strains clustered with the V. campbellii type and one reference strain; seven strains clustered with the V. harveyi type and three reference strains; and the type and four reference strains of V. rotiferianus grouped together. The correlations between DNA–DNA hybridization and the genomic fingerprinting by FAFLP and (GTG)₅-PCR were found to be above 0·68 and statistically significant, suggesting the value of the latter techniques for the reliable identification of V. harveyi-related species. The results presented indicate that strains phenotypically identified as V. harveyi are in fact V. campbellii; these findings position V. campbellii as an important species involved in diseases of reared aquatic organisms.

This article has been cited by other articles:

				F. L. Thompson, B. Gomez-Gil, A. T. R. Vasconcelos, and T. Sawabe Multilocus Sequence Analysis Reveals that Vibrio harveyi and V. campbellii Are Distinct Species Appl. Envir. Microbiol., July 1, 2007; 73(13): 4279 - 4285. [Abstract] [Full Text] [PDF]

				T. Defoirdt, R. Crab, T. K. Wood, P. Sorgeloos, W. Verstraete, and P. Bossier Quorum Sensing-Disrupting Brominated Furanones Protect the Gnotobiotic Brine Shrimp Artemia franciscana from Pathogenic Vibrio harveyi, Vibrio campbellii, and Vibrio parahaemolyticus Isolates Appl. Envir. Microbiol., September 1, 2006; 72(9): 6419 - 6423. [Abstract] [Full Text] [PDF]

				F. L. Thompson, D. Gevers, C. C. Thompson, P. Dawyndt, S. Naser, B. Hoste, C. B. Munn, and J. Swings Phylogeny and Molecular Identification of Vibrios on the Basis of Multilocus Sequence Analysis Appl. Envir. Microbiol., September 1, 2005; 71(9): 5107 - 5115. [Abstract] [Full Text] [PDF]

				A. Marques, T. Dinh, C. Ioakeimidis, G. Huys, J. Swings, W. Verstraete, J. Dhont, P. Sorgeloos, and P. Bossier Effects of Bacteria on Artemia franciscana Cultured in Different Gnotobiotic Environments Appl. Envir. Microbiol., August 1, 2005; 71(8): 4307 - 4317. [Abstract] [Full Text] [PDF]

				F. L. Thompson, T. Iida, and J. Swings Biodiversity of Vibrios Microbiol. Mol. Biol. Rev., September 1, 2004; 68(3): 403 - 431. [Abstract] [Full Text] [PDF]