| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Integrated Genomics, Inc., 2201 West Campbell Park Dr., Chicago, IL 60612, USA
2 Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, ID 83843, USA
3 The Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridge CB10 1RQ, UK
4 Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, IL 60612-7344, USA
Correspondence
Birgit M. Prüß
preuss{at}uic.edu
or
BirgitPruess{at}ndsu.nodak.edu
This paper focuses on global gene regulation by FlhD/FlhC in enteric bacteria. Even though Yersinia enterocolitica FlhD/FlhC can complement an Escherichia coli flhDC mutant for motility, it is not known if the Y. enterocolitica FlhD/FlhC complex has an effect on metabolism similar to E. coli. To study metabolic gene regulation, a partial Yersinia enterocolitica 8081c microarray was constructed and the expression patterns of wild-type cells were compared to an flhDC mutant strain at 25 and 37 °C. The overlap between the E. coli and Y. enterocolitica FlhD/FlhC regulated genes was 25 %. Genes that were regulated at least fivefold by FlhD/FlhC in Y. enterocolitica are genes encoding urocanate hydratase (hutU), imidazolone propionase (hutI), carbamoylphosphate synthetase (carAB) and aspartate carbamoyltransferase (pyrBI). These enzymes are part of a pathway that is involved in the degradation of L-histidine to L-glutamate and eventually leads into purine/pyrimidine biosynthesis via carbamoylphosphate and carbamoylaspartate. A number of other genes were regulated at a lower rate. In two additional experiments, the expression of wild-type cells grown at 4 or 25 °C was compared to the same strain grown at 37 °C. The expression of the flagella master operon flhD was not affected by temperature, whereas the flagella-specific sigma factor fliA was highly expressed at 25 °C and reduced at 4 and 37 °C. Several other flagella genes, all of which are under the control of FliA, exhibited a similar temperature profile. These data are consistent with the hypothesis that temperature regulation of flagella genes might be mediated by the flagella-specific sigma factor FliA and not the flagella master regulator FlhD/FlhC.
Present address: Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo, ND 58105, USA.
This article has been cited by other articles:
|
|
 |
|
  A. Chatterjee, Y. Cui, and A. K. Chatterjee RsmC of Erwinia carotovora subsp. carotovora Negatively Controls Motility, Extracellular Protein Production, and Virulence by Binding FlhD and Modulating Transcriptional Activity of the Master Regulator, FlhDC J. Bacteriol., July 15, 2009; 191(14): 4582 - 4593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Cui, A. Chatterjee, H. Yang, and A. K. Chatterjee Regulatory Network Controlling Extracellular Proteins in Erwinia carotovora subsp. carotovora: FlhDC, the Master Regulator of Flagellar Genes, Activates rsmB Regulatory RNA Production by Affecting gacA and hexA (lrhA) Expression J. Bacteriol., July 1, 2008; 190(13): 4610 - 4623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Bresolin, J. Trcek, S. Scherer, and T. M. Fuchs Presence of a functional flagellar cluster Flag-2 and low-temperature expression of flagellar genes in Yersinia enterocolitica W22703 Microbiology, January 1, 2008; 154(1): 196 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Claret, S. Miquel, N. Vieille, D. A. Ryjenkov, M. Gomelsky, and A. Darfeuille-Michaud The Flagellar Sigma Factor FliA Regulates Adhesion and Invasion of Crohn Disease-associated Escherichia coli via a Cyclic Dimeric GMP-dependent Pathway J. Biol. Chem., November 16, 2007; 282(46): 33275 - 33283. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Perez-Gutierrez, C. M. Llompart, M. Skurnik, and J. A. Bengoechea Expression of the Yersinia enterocolitica pYV-Encoded Type III Secretion System Is Modulated by Lipopolysaccharide O-Antigen Status Infect. Immun., March 1, 2007; 75(3): 1512 - 1516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. P. Stafford, T. Ogi, and C. Hughes Binding and transcriptional activation of non-flagellar genes by the Escherichia coli flagellar master regulator FlhD2C2 Microbiology, June 1, 2005; 151(6): 1779 - 1788. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
