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Microbiology 150 (2004), 2409-2414; DOI  10.1099/mic.0.27106-0
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Microbiology 150 (2004), 2409-2414; DOI  10.1099/mic.0.27106-0
© 2004 Society for General Microbiology

rexAB mutants in Streptococcus pneumoniae

David Halpern1, Alexandra Gruss1, Jean-Pierre Claverys2 and Meriem El Karoui1

1 Unité de Recherches Laitières et Génétique Appliquée, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas, France
2 Laboratoire de Microbiologie et Génétique Moléculaires UMR 5100, CNRS Université Paul Sabatier, 118, route de Narbonne, 31062 Toulouse cedex, France

Correspondence
Meriem El Karoui
meriem{at}diamant.jouy.inra.fr

Streptococcus pneumoniae is a human pathogen that is naturally transformable. In this study a major component of the homologous recombination pathway, the RexAB exonuclease/helicase, was characterized. rexA and rexB insertional mutants were constructed using mariner mutagenesis and found to have identical phenotypes. Both rexAB mutants displayed poor cell viability, reduced double-strand exonuclease activity, UV sensitivity and a reduced level of gene conversion compared to the wild-type strain. No effect was observed on plasmid and chromosomal transformation efficiencies. These results indicate that in S. pneumoniae, RexAB is required for DNA repair, but not for chromosomal transformation and plasmid establishment.

Abbreviations: CFC, colony-forming centres; DSB, double-strand DNA break; HMW, high molecular weight; Spc, spectinomycin; Str, streptomycin; wt, wild-type




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