HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Pina, C. Articles by Loureiro-Dias, M. C. Search for Related Content PubMed PubMed Citation Articles by Pina, C. Articles by Loureiro-Dias, M. C. Agricola Articles by Pina, C. Articles by Loureiro-Dias, M. C.

Ffz1, a new transporter specific for fructose from Zygosaccharomyces bailii

¹ Dep. Botânica e Engenharia Biológica, Instituto Superior de Agronomia, 1349-017 Lisboa, Portugal
² Centro de Recursos Microbiológicos, SABT, FCT/UNL, 2825-516 Caparica, Portugal

Correspondence
Maria C. Loureiro-Dias
mcdias{at}isa.utl.pt

The basis of fructophily in the yeast Zygosaccharomyces bailii has been shown to reside in the performance of transport systems for hexoses. In this study, a gene encoding a fructose-specific transporter was characterized. The strategy involved the functional complementation of a Saccharomyces cerevisiae strain that does not take up hexoses (hxt-null strain). This strain was transformed with a genomic library of Z. bailii. One transformant capable of growing on fructose, but not on glucose, was obtained. This transformant did not transport D-[¹⁴C]glucose, and the kinetic parameters for D-[¹⁴C]fructose were V_max=3·3 mmol h^–1 g^–1 and K_m=80·4 mM. As in the original strain of Z. bailii, fructose uptake was not inhibited by the presence of other hexoses or uranyl. The plasmid responsible for the observed phenotype was found to carry an ORF encoding a 616 amino acid protein with the characteristics of a membrane transporter, which was designated FFZ1 (fructose facilitator Zygosaccharomyces). The impairment in function observed in an S. cerevisiae transformant expressing a truncated Ffz1 protein lacking 67 amino acids at the C-terminus suggests an important role for this terminal part in the proper structure of the transporter.

This article has been cited by other articles:

				J. Santos, M. J. Sousa, H. Cardoso, J. Inacio, S. Silva, I. Spencer-Martins, and C. Leao Ethanol tolerance of sugar transport, and the rectification of stuck wine fermentations Microbiology, February 1, 2008; 154(2): 422 - 430. [Abstract] [Full Text] [PDF]